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Literature summary for 4.1.3.40 extracted from

  • Pfaff, C.; Glindemann, N.; Gruber, J.; Frentzen, M.; Sadre, R.
    Chorismate pyruvate-lyase and 4-hydroxy-3-solanesylbenzoate decarboxylase are required for plastoquinone biosynthesis in the cyanobacterium Synechocystis sp. PCC6803 (2014), J. Biol. Chem., 289, 2675-2686.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.40

Cloned(Commentary)

Cloned (Comment) Organism
gene sll1797, phylogenetic analysis and tree, expression in Escherichia coli strain BL21AI as GST-tagged protein, and in Escherichia coli strain BW25113 for quantitative expression analysis by real time quantitative PCR. Complementation of ubiquinone-deficient Escherichia coli mutants by catalytic activity of heterologous Sll1797 Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information generation of a Synechocystis sp. single-gene knockout mutant lacking the chorismate pyruvate-lyase Sll1797 Synechocystis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the recombinant enzyme shows severalfold higher specific activities in membrane than in soluble protein fractions Synechocystis sp. 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chorismate Synechocystis sp.
-
 4-hydroxybenzoate + pyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. P73322 gene sll1797
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21AI by glutathione affinity chromatography Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate
-
 Synechocystis sp. 4-hydroxybenzoate + pyruvate
-
 ?

Synonyms

Synonyms Comment Organism
chorismate pyruvate-lyase
-
 Synechocystis sp.
Sll1797
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Synechocystis sp.

General Information

General Information Comment Organism
malfunction a knockout mutant shows highyl reduced plastoquinone content, and the mutant requires 4-hydroxybenzoate for growth Synechocystis sp.
metabolism the enzyme is required for initiation of cyanobacterial plastoquinone biosynthesis, together with two 4-hydroxy-3-solanesylbenzoate decarboxylases. The chorismate pyruvate-lyase Sll1797 catalyzes the conversion of chorismate to 4-hydroxybenzoate that is prenylated by Slr0926. Slr1099 and Sll0936 are both involved in the decarboxylation of 4-hydroxy-3-solanesylbenzoate Synechocystis sp.