Cloned (Comment) | Organism |
---|---|
cloning of 3-hydroxy-3-methylglutaryl-CoA lyase-like protein, sequence comparison of HMGCLL1 and HMGCL, expression of 3-hydroxy-3-methylglutaryl-CoA lyase-like protein mutant G2A in COS-1 cell cytosol, recombinant expression of wild-type 3-hydroxy-3-methylglutaryl-CoA lyase-like protein in Escherichia coli and Pichia pastoris, in insoluble form | Homo sapiens |
sequence comparison of HMGCLL1 and HMGCL, expression of 3-hydroxy-3-methylglutaryl-CoA lyase wild-type in COS-1 cell cytosol, recombinant expression of wild-type 3-hydroxy-3-methylglutaryl-CoA lyase in Escherichia coli and Pichia pastoris, in active form | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C323S | site-directed mutagenesis of HMGCL, the mutant shows 2fold increased activity compared to wild-type HMGCL | Homo sapiens |
G2A | site-directed mutagenesis of HMGCLL1, eliminatin of N-terminal myristoylation motif by construction of a 3-hydroxy-3-methylglutaryl-CoA lyase-like protein HMGCLL1 G2A mutant, the mutant shows reduced activity compared to wild-type HMGCLL1 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | HMGCLL1 mutant G2A and wild-type HMGCL, pH 8.2, 37°C | Homo sapiens | |
0.024 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | wild-type HMGCL, pH 8.2, 37°C | Homo sapiens | |
0.028 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | wild-type HMGCLL1, pH 8.2, 37°C | Homo sapiens | |
0.045 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | wild-type HMGCLL1, pH 8.2, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi-derived vesicle | endogenous HMGCLL1 localized to punctate vesicles | Homo sapiens | - |
- |
mitochondrion | 3-hydroxy-3-methylglutaryl-CoA lyase | Homo sapiens | 5739 | - |
additional information | HMGCL is a mitochondrial enzyme | Homo sapiens | - |
- |
additional information | HMGCLL1 is an extramitochondrial enzyme | Homo sapiens | - |
- |
perinuclear space | wild-type 3-hydroxy-3-methylglutaryl-CoA lyase-like protein, myristoylation dependent association with nonmitochondrial membrane compartments | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, Km for wild-type HMGCL is 0.233 mM and for HMGCL mutant C323A 0.322 mM | Homo sapiens | |
Mg2+ | required, Km of wild-type HMGCLL1 is 0.049 mM and for HMGCLL1 mutant G2A 0.088 mM | Homo sapiens | |
Mn2+ | required, Km for wild-type HMGCL is 0.00034 mM and for HMGCL mutant C323A 0.00037 mM | Homo sapiens | |
Mn2+ | required, Km of wild-type HMGCLL1 is 0.00018 mM and for HMGCLL1 mutant G2A 0.00024 mM | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-Hydroxy-3-methylglutaryl-CoA | Homo sapiens | - |
Acetyl-CoA + acetoacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | the deduced 3-hydroxy-3-methylglutaryl-CoA lyase-like protein, HMGCLL1, sequence contains an N-terminal myristoylation motif. Myristoylation of HMGCLL1 affects its cellular localization | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant active wild-type 3-hydroxy-3-methylglutaryl-CoA lyase-like protein and its mutant G2A from Pichia pastoris by nickel affinity chromatography and ultrafiltration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | HMGCL | Homo sapiens | - |
brain cancer cell | HMGCLL1 is overexpressed in certain types of human brain cancer cells | Homo sapiens | - |
cecum | HMGCL | Homo sapiens | - |
duodenum | HMGCL | Homo sapiens | - |
duodenum | HMGCLL1 | Homo sapiens | - |
glioblastoma cell | HMGCLL1 | Homo sapiens | - |
kidney | HMGCL | Homo sapiens | - |
large intestine | HMGCL | Homo sapiens | - |
liver | HMGCL | Homo sapiens | - |
additional information | tissue distribution of 3-hydroxy-3-methylglutaryl-CoA lyase, overview | Homo sapiens | - |
additional information | tissue distribution of 3-hydroxy-3-methylglutaryl-CoA lyase-like protein, overview | Homo sapiens | - |
small intestine | HMGCL | Homo sapiens | - |
small intestine | HMGCLL1 | Homo sapiens | - |
U-87MG cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
117 | - |
HMGCLL1 mutant G2A, pH 8.2, 37°C | Homo sapiens |
150 | - |
wild-type HMGCLL1, pH 8.2, 37°C | Homo sapiens |
159 | - |
wild-type HMGCL, pH 8.2, 37°C | Homo sapiens |
348 | - |
HMGCL mutant C323A, pH 8.2, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Homo sapiens | Acetyl-CoA + acetoacetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-Hydroxy-3-methylglutaryl-CoA lyase | - |
Homo sapiens |
3-hydroxy-3-methylglutaryl-CoA lyase-like protein | - |
Homo sapiens |
HMG-CoA lyase | - |
Homo sapiens |
HMGCL | - |
Homo sapiens |
HMGCLL1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Homo sapiens |