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Literature summary for 4.1.3.4 extracted from
- Effect of polyphenols on 3-hydroxy-3-methylglutaryl-coenzyme A lyase activity in human hepatoma HepG2 cell extracts (2013), Biol. Pharm. Bull., 36, 1902-1906.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| daidzein | slight activation | Homo sapiens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (-)-epicatechin gallate | a mixed-type noncompetitive inhibitor | Homo sapiens | |
| (-)-epigallocatechin | a mixed-type noncompetitive inhibitor | Homo sapiens | |
| (-)-epigallocatechin gallate | a mixed-type noncompetitive inhibitor, contribution of the gallyl moiety, which forms a hexa-coordination complex with the Mg2+ ion, to enzymatic inhibition | Homo sapiens | |
| gallic acid | a noncompetitive inhibitor | Homo sapiens | |
| additional information | no inhibition by daidzein, hesperetin, naringenin, (+)-catechin, and (-)-epicatechin | Homo sapiens | |
| pyrogallol | a mixed-type noncompetitive inhibitor | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Hep-G2 cell | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Homo sapiens | Acetyl-CoA + acetoacetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-hydroxy-3-methylglutaryl-coenzyme A lyase | - |
Homo sapiens |
| HMG-CoA lyase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
(-)-epigallocatechin gallate | pH 7.5, 37°C | Homo sapiens | |
| 0.0074 | - |
pyrogallol | pH 7.5, 37°C | Homo sapiens | |
| 0.018 | - |
gallic acid | pH 7.5, 37°C | Homo sapiens | |
| 0.0299 | - |
(-)-epigallocatechin | pH 7.5, 37°C | Homo sapiens | |
| 0.0441 | - |
(-)-epicatechin gallate | pH 7.5, 37°C | Homo sapiens | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0424 | - |
pH 7.5, 37°C | Homo sapiens | (-)-epigallocatechin gallate | |
| 0.0444 | - |
pH 7.5, 37°C | Homo sapiens | gallic acid | |
| 0.0575 | - |
pH 7.5, 37°C | Homo sapiens | (-)-epigallocatechin | |
| 0.0615 | - |
pH 7.5, 37°C | Homo sapiens | pyrogallol | |
| 0.1612 | - |
pH 7.5, 37°C | Homo sapiens | (-)-epicatechin gallate | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inhibition of this enzyme results in reduced acetoacetate production and impaired ketoacidosis. High levels of secreted ketone bodies (acetoacetate and 3beta-hydroxybutyrate) lower the pH of blood and urine, resulting in ketoacidosis | Homo sapiens |
| physiological function | the enzyme is a rate-limiting enzyme in hepatic cells catalyzing the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetate | Homo sapiens |
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