Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
overexpression of wild-type enzyme and synthetic C323 mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | C323 involved in intersubunit cross-linking upon oxidative inactivation, thiol-disulfide exchange mechanism | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
hydroxymethylglutaryl-CoA | wild-type | Homo sapiens | |
0.045 | - |
hydroxymethylglutaryl-CoA | C323S mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31600 | - |
x * 31600, estimated from nucleotide sequence | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and C323S mutant | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-3-Hydroxy-3-methylglutaryl-CoA = acetyl-CoA + acetoacetate | identification of reactive cysteines, possibly regulatory mechanism via thiol/disulfide exchange | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
159 | - |
wild-type | Homo sapiens |
348 | - |
C323S mutant | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA | - |
Homo sapiens | acetoacetate + acetyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31600, estimated from nucleotide sequence | Homo sapiens |
More | C323 involved in intersubunit cross-linking upon oxidative inactivation | Homo sapiens |