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Literature summary for 4.1.3.4 extracted from

  • Roberts, J.R.; Narasimhan, C.; Hruz, P.W.; Mitchell, G.A.; Miziorko, H.M.
    3-Hydroxy-3-methylglutaryl-CoA lyase: expression and isolation of the recombinant human enzyme and investigation of a mechanism for regulation of enzyme activity (1994), J. Biol. Chem., 269, 17841-17846.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens
overexpression of wild-type enzyme and synthetic C323 mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information C323 involved in intersubunit cross-linking upon oxidative inactivation, thiol-disulfide exchange mechanism Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
hydroxymethylglutaryl-CoA wild-type Homo sapiens
0.045
-
hydroxymethylglutaryl-CoA C323S mutant Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens
Mn2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31600
-
x * 31600, estimated from nucleotide sequence Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme and C323S mutant Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
(S)-3-Hydroxy-3-methylglutaryl-CoA = acetyl-CoA + acetoacetate identification of reactive cysteines, possibly regulatory mechanism via thiol/disulfide exchange Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
159
-
wild-type Homo sapiens
348
-
C323S mutant Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA
-
Homo sapiens acetoacetate + acetyl-CoA
-
?

Subunits

Subunits Comment Organism
? x * 31600, estimated from nucleotide sequence Homo sapiens
More C323 involved in intersubunit cross-linking upon oxidative inactivation Homo sapiens