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Literature summary for 4.1.3.4 extracted from
- Purification and characterization of avian liver 3-hydroxy-3-methylglutaryl coenzyme A lyase (1980), J. Biol. Chem., 255, 11023-11028.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thiols | - |
Anas sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
hydroxymethylglutaryl-CoA | - |
Anas sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | matrix | Anas sp. | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 22% activation at 0.1 mM | Anas sp. | |
| Cu2+ | 37% activation at 0.1 mM | Anas sp. | |
| Mg2+ | 330% activation at 0.1 mM | Anas sp. | |
| Mn2+ | 290% activation at 0.1 mM | Anas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
2 * 27000, SDS-PAGE | Anas sp. |
| 49000 | - |
gel filtration | Anas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anas sp. | - |
duck | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Anas sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Anas sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 351 | - |
- |
Anas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C,Tris-HCl buffer, pH 7.1, 30% glycerol, 1 mM dithiothreitol, 6 weeks | Anas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Anas sp. | Acetyl-CoA + acetoacetate | - |
? | |
| 3-hydroxy-3-methylglutaryl-CoA | - |
Anas sp. | acetoacetate + acetyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 27000, SDS-PAGE | Anas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme assay at | Anas sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 158 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | - |
Anas sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
- |
Anas sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Anas sp. |
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Release 2023.1 (January 2023)
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