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Literature summary for 4.1.3.39 extracted from

  • Baker, P.; Carere, J.; Seah, S.Y.
    Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI (2011), Biochemistry, 50, 3559-3569.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(lambdaDE3) Paraburkholderia xenovorans

Protein Variants

Protein Variants Comment Organism
H20A decrease in kcat/Km value Paraburkholderia xenovorans
H20S decrease in kcat/Km value Paraburkholderia xenovorans
L87A 3-fold reduction in kcat/Km Paraburkholderia xenovorans
L87A/L89A improved specificity for aldehydes at least four carbons in length Paraburkholderia xenovorans
L89A kcat/Km similar to wild type Paraburkholderia xenovorans
R16A no detectable activity Paraburkholderia xenovorans
R16K increased Km value for 4-hydroxy-2-oxopentanoate Paraburkholderia xenovorans
Y290F lower kcat value Paraburkholderia xenovorans
Y290S lower kcat value Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
(4R)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.013
-
(4S)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.013
-
(4R)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.013
-
(4S)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.089
-
(4S)-4-hydroxy-2-oxopentanoate wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.222
-
(4S)-4-hydroxy-2-oxopentanoate H20A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.228
-
(4S)-4-hydroxy-2-oxopentanoate H20S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
11
-
pyruvate aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
13
-
pyruvate aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
16.8
-
propionaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
20.2
-
pyruvate aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
24.8
-
pentaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
33.3
-
pentaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
40.2
-
propionaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
49.2
-
Butyraldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
64.2
-
acetaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
80.5
-
acetaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
136
-
propionaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
289
-
Butyraldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
323
-
Butyraldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4R)-4-hydroxy-2-oxopentanoate
-
Paraburkholderia xenovorans acetaldehyde + pyruvate
-
r
(4S)-4-hydroxy-2-oxopentanoate
-
Paraburkholderia xenovorans acetaldehyde + pyruvate
-
r
butyraldehyde + pyruvate
-
Paraburkholderia xenovorans 4-hydroxy-2-oxoheptanoate
-
r
hexaldehyde + pyruvate
-
Paraburkholderia xenovorans 4-hydroxy-2-oxononanoate
-
r
pentaldehyde + pyruvate
-
Paraburkholderia xenovorans 4-hydroxy-2-oxooctanoate
-
r
propionaldehyde + pyruvate
-
Paraburkholderia xenovorans 4-hydroxy-2-oxohexanoate
-
r

Synonyms

Synonyms Comment Organism
class II pyruvate aldolase BphI
-
Paraburkholderia xenovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.089
-
(4S)-4-hydroxy-2-oxopentanoate H20S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.099
-
(4S)-4-hydroxy-2-oxopentanoate H20A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.13
-
pentaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.131
-
(4R)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.132
-
(4S)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.17
-
(4R)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.171
-
(4S)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.26
-
Butyraldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.29
-
propionaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.3
-
Butyraldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.32
-
pyruvate aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.58
-
pentaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.63
-
propionaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.64
-
pyruvate aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.64
-
Butyraldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.86
-
acetaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.94
-
acetaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
1.2
-
pyruvate aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
1.79
-
propionaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.07
-
(4S)-4-hydroxy-2-oxopentanoate wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Paraburkholderia xenovorans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5
-
Paraburkholderia xenovorans

General Information

General Information Comment Organism
physiological function polychlorinated biphenyl degradation pathway Paraburkholderia xenovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00001
-
hexaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00001
-
hexaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00001
-
hexaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00013
-
hexaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00033
-
pentaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00042
-
acetaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00047
-
pentaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00049
-
acetaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00079
-
Butyraldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00104
-
Butyraldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00219
-
Butyraldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0038
-
pentaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.00673
-
propionaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0117
-
acetaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.012
-
(4S)-4-hydroxy-2-oxopentanoate R16K mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0129
-
Butyraldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0132
-
propionaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0134
-
acetaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0156
-
propionaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0159
-
pyruvate aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0175
-
propionaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0233
-
pentaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0587
-
pyruvate aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.0915
-
pyruvate aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.34
-
(4S)-4-hydroxy-2-oxopentanoate H20S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
0.45
-
(4S)-4-hydroxy-2-oxopentanoate H20A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
10.4
-
(4R)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
12.6
-
(4S)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
13.4
-
(4S)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
14.1
-
(4R)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
44.7
-
(4S)-4-hydroxy-2-oxopentanoate wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans