Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(lambdaDE3) | Paraburkholderia xenovorans |
Protein Variants | Comment | Organism |
---|---|---|
H20A | decrease in kcat/Km value | Paraburkholderia xenovorans |
H20S | decrease in kcat/Km value | Paraburkholderia xenovorans |
L87A | 3-fold reduction in kcat/Km | Paraburkholderia xenovorans |
L87A/L89A | improved specificity for aldehydes at least four carbons in length | Paraburkholderia xenovorans |
L89A | kcat/Km similar to wild type | Paraburkholderia xenovorans |
R16A | no detectable activity | Paraburkholderia xenovorans |
R16K | increased Km value for 4-hydroxy-2-oxopentanoate | Paraburkholderia xenovorans |
Y290F | lower kcat value | Paraburkholderia xenovorans |
Y290S | lower kcat value | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
(4R)-4-hydroxy-2-oxopentanoate | Y290S mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.013 | - |
(4S)-4-hydroxy-2-oxopentanoate | Y290F mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.013 | - |
(4R)-4-hydroxy-2-oxopentanoate | Y290F mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.013 | - |
(4S)-4-hydroxy-2-oxopentanoate | Y290S mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.089 | - |
(4S)-4-hydroxy-2-oxopentanoate | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.222 | - |
(4S)-4-hydroxy-2-oxopentanoate | H20A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.228 | - |
(4S)-4-hydroxy-2-oxopentanoate | H20S mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
11 | - |
pyruvate | aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
13 | - |
pyruvate | aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
16.8 | - |
propionaldehyde | L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
20.2 | - |
pyruvate | aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
24.8 | - |
pentaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
33.3 | - |
pentaldehyde | L87A/L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
40.2 | - |
propionaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
49.2 | - |
Butyraldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
64.2 | - |
acetaldehyde | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
80.5 | - |
acetaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
136 | - |
propionaldehyde | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
289 | - |
Butyraldehyde | L87A/L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
323 | - |
Butyraldehyde | L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paraburkholderia xenovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-4-hydroxy-2-oxopentanoate | - |
Paraburkholderia xenovorans | acetaldehyde + pyruvate | - |
r | |
(4S)-4-hydroxy-2-oxopentanoate | - |
Paraburkholderia xenovorans | acetaldehyde + pyruvate | - |
r | |
butyraldehyde + pyruvate | - |
Paraburkholderia xenovorans | 4-hydroxy-2-oxoheptanoate | - |
r | |
hexaldehyde + pyruvate | - |
Paraburkholderia xenovorans | 4-hydroxy-2-oxononanoate | - |
r | |
pentaldehyde + pyruvate | - |
Paraburkholderia xenovorans | 4-hydroxy-2-oxooctanoate | - |
r | |
propionaldehyde + pyruvate | - |
Paraburkholderia xenovorans | 4-hydroxy-2-oxohexanoate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
class II pyruvate aldolase BphI | - |
Paraburkholderia xenovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
(4S)-4-hydroxy-2-oxopentanoate | H20S mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.099 | - |
(4S)-4-hydroxy-2-oxopentanoate | H20A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.13 | - |
pentaldehyde | L87A/L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.131 | - |
(4R)-4-hydroxy-2-oxopentanoate | Y290F mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.132 | - |
(4S)-4-hydroxy-2-oxopentanoate | Y290F mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.17 | - |
(4R)-4-hydroxy-2-oxopentanoate | Y290S mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.171 | - |
(4S)-4-hydroxy-2-oxopentanoate | Y290S mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.26 | - |
Butyraldehyde | L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.29 | - |
propionaldehyde | L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.3 | - |
Butyraldehyde | L87A/L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.32 | - |
pyruvate | aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.58 | - |
pentaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.63 | - |
propionaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.64 | - |
pyruvate | aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.64 | - |
Butyraldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.86 | - |
acetaldehyde | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.94 | - |
acetaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
1.2 | - |
pyruvate | aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
1.79 | - |
propionaldehyde | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.07 | - |
(4S)-4-hydroxy-2-oxopentanoate | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Paraburkholderia xenovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Paraburkholderia xenovorans |
General Information | Comment | Organism |
---|---|---|
physiological function | polychlorinated biphenyl degradation pathway | Paraburkholderia xenovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
hexaldehyde | L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00001 | - |
hexaldehyde | L87A/L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00001 | - |
hexaldehyde | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00013 | - |
hexaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00033 | - |
pentaldehyde | L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00042 | - |
acetaldehyde | L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00047 | - |
pentaldehyde | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00049 | - |
acetaldehyde | L87A/L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00079 | - |
Butyraldehyde | L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00104 | - |
Butyraldehyde | L87A/L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00219 | - |
Butyraldehyde | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0038 | - |
pentaldehyde | L87A/L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.00673 | - |
propionaldehyde | L87A/L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0117 | - |
acetaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.012 | - |
(4S)-4-hydroxy-2-oxopentanoate | R16K mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0129 | - |
Butyraldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0132 | - |
propionaldehyde | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0134 | - |
acetaldehyde | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0156 | - |
propionaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0159 | - |
pyruvate | aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0175 | - |
propionaldehyde | L87A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0233 | - |
pentaldehyde | L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0587 | - |
pyruvate | aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.0915 | - |
pyruvate | aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.34 | - |
(4S)-4-hydroxy-2-oxopentanoate | H20S mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.45 | - |
(4S)-4-hydroxy-2-oxopentanoate | H20A mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
10.4 | - |
(4R)-4-hydroxy-2-oxopentanoate | Y290F mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
12.6 | - |
(4S)-4-hydroxy-2-oxopentanoate | Y290F mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
13.4 | - |
(4S)-4-hydroxy-2-oxopentanoate | Y290S mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
14.1 | - |
(4R)-4-hydroxy-2-oxopentanoate | Y290S mutant protein, pH 8.0, 25°C | Paraburkholderia xenovorans | |
44.7 | - |
(4S)-4-hydroxy-2-oxopentanoate | wild type protein, pH 8.0, 25°C | Paraburkholderia xenovorans |