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Literature summary for 4.1.3.30 extracted from
- Gene acquisition, duplication and metabolic specification: the evolution of fungal methylisocitrate lyases (2011), Environ. Microbiol., 13, 1534-1548.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged version expressed in Escherichia coli BL21(DE3)Rosetta2 | Aspergillus fumigatus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L521F | L521 contributes to substrate specificity | Aspergillus fumigatus |
| L521F/S523T | L521 and S523 contribute to substrate specificity | Aspergillus fumigatus |
| S523T | S523 contributes to substrate specificity | Aspergillus fumigatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| isocitrate | - |
Aspergillus fumigatus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0181 | - |
methylisocitrate | wild type protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
| 0.0485 | - |
methylisocitrate | L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
| 0.0674 | - |
methylisocitrate | S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
| 0.0806 | - |
methylisocitrate | L521F mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Aspergillus fumigatus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
| Aspergillus fumigatus Af293 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+) | Aspergillus fumigatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity not detectable for substrate isocitrate and wild type protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
| 0.00055 | - |
substrate isocitrate, S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
| 0.00105 | - |
substrate isocitrate, L521F mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
| 0.0033 | - |
substrate isocitrate, L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
| 4.33 | - |
L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
| 7.52 | - |
S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
| 27.43 | - |
L521F mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
| 32.33 | - |
wild type protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methylisocitrate | - |
Aspergillus fumigatus | succinate + pyruvate | - |
? | |
| methylisocitrate | - |
Aspergillus fumigatus Af293 | succinate + pyruvate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methylisocitrate lyase | - |
Aspergillus fumigatus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.258 mM isocitrate required to double the KM-value with methylisocitrate (wild type protein), 0.0598 mM isocitrate required to double the KM-value with methylisocitrate (L521F mutant protein), 0.0606 mM isocitrate required to double the KM-value with methylisocitrate (S523T mutant protein), 0.0119 mM isocitrate required to double the KM-value with methylisocitrate (L521F/L521F mutant protein) | Aspergillus fumigatus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 92.8 | - |
methylisocitrate | L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
| 116 | - |
methylisocitrate | S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
| 353 | - |
methylisocitrate | L521F mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
| 1850 | - |
methylisocitrate | wild type protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
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