Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 5 mM, required for optimal activity | Salmonella enterica | |
glutathione | required for optimal activity | Salmonella enterica |
Cloned (Comment) | Organism |
---|---|
- |
Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
C123A | no enzyme activity | Salmonella enterica |
D58A | no enzyme activity | Salmonella enterica |
H125A | decreased kcat | Salmonella enterica |
K121A | decreased kcat | Salmonella enterica |
R122K | decreased kcat | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7.5, 37°C, H125A mutant | Salmonella enterica | |
19 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7.5, 37°C, wild-type | Salmonella enterica | |
22 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7.5, 37°C, R122K mutant | Salmonella enterica | |
57 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7.5, 37°C, K121A mutant | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Salmonella enterica | enzyme in methylcitrate cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
serovar typhimurium LT2 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = succinate + pyruvate | mechanism | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | syn: methylisocitrate | Salmonella enterica | Pyruvate + succinate | - |
? | |
additional information | no substrate: isocitrate | Salmonella enterica | ? | - |
? | |
additional information | enzyme in methylcitrate cycle | Salmonella enterica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
PrpB | - |
Salmonella enterica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Salmonella enterica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
14% activity after 10 min | Salmonella enterica |
60 | - |
no detectable activity | Salmonella enterica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7.5, 37°C, K121A mutant | Salmonella enterica | |
0.14 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7.5, 37°C, H125A mutant | Salmonella enterica | |
38 | - |
Mg2+ | pH 7.5, 37°C | Salmonella enterica | |
55 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7.5, 37°C, R122K mutant | Salmonella enterica | |
105 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7.5, 37°C, wild-type | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Salmonella enterica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | 16% activity at pH 6, 96% activity at pH 8.5 | Salmonella enterica |