General Stability | Organism |
---|---|
enzyme becomes inactivated when incubated with oxygen, partial reactivation with dithiothreitol possible | Aspergillus nidulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Bromopyruvate | complete inactivation at equimolar concentrations | Aspergillus nidulans | |
Ca2+ | 40% inactivation at 2 mM; inhibition at 2 mM | Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7 | Aspergillus nidulans | |
31 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7 | Aspergillus nidulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent, 2 mM, Km: 58 mM at pH 7 | Aspergillus nidulans | |
Mg2+ | dependentm Km: 35 mM at pH 7 | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Aspergillus nidulans |
66000 | - |
4 * 66000, SDS-PAGE | Aspergillus nidulans |
130000 | - |
gel filtration | Aspergillus nidulans |
244000 | - |
gel filtration | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aspergillus nidulans | enzyme in methylcitrate cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Oxidation Stability | Organism |
---|---|
enzyme becomes inactivated when incubated with oxygen, partial reactivation with dithiothreitol possible | Aspergillus nidulans |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus nidulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.4 | - |
- |
Aspergillus nidulans |
91 | - |
- |
Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | syn: methylisocitrate, enzyme acts only on threo-diastereomer, but not on the erythro-diastereomer | Aspergillus nidulans | Pyruvate + succinate | - |
? | |
additional information | no substrate: isocitrate | Aspergillus nidulans | ? | - |
? | |
additional information | enzyme in methylcitrate cycle | Aspergillus nidulans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 66000, SDS-PAGE | Aspergillus nidulans |
tetramer | 4 * 32000, SDS-PAGE | Aspergillus nidulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
stable for several days | Aspergillus nidulans |
40 | - |
stable for 60 min | Aspergillus nidulans |
50 | - |
half-life: 15 min | Aspergillus nidulans |
50 | - |
complete inactivation after 15 min | Aspergillus nidulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.3 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7 | Aspergillus nidulans | |
100 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | pH 7 | Aspergillus nidulans |