Literature summary for 4.1.3.3 extracted from

Zhang, Y.; Tao, F.; Du, M.; Ma, C.; Qiu, J.; Gu, L.; He, X.; Xu, P.
An efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system (2010), Appl. Microbiol. Biotechnol., 86, 481-489.

Search for more literature for 4.1.3.3

Application

Application	Comment	Organism
biotechnology	efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system, a precursor for producing many pharmaceutical drugs such as zanamivir which have been used in clinical trials to treat and prevent the infection with influenza virus, such as the avian influenza virus H5N1 and the current 2009 H1N1	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
2 recombinant Escherichia coli strains capable of expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid aldolase are constructed based on a highly efficient temperature-responsive expression system which is safe compared to chemical-induced systems and coupled in N-acetyl-D-neuraminic acid production	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	determined by SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-acetyl-D-neuraminic acid	Escherichia coli	-	N-acetyl-D-mannosamine + pyruvate	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	after a 36 h incubation, the concentrations of N-acetyl-D-neuraminic acid from the reactions utilizing 0.4, 0.8, 1.2, 1.6, 2.0, and 2.2 M pyruvate as the carbon source are 7.5 (3.75%), 26.9 (3.45%), 43.9 (21.95%), 56.0 (28%), and 61.9 mM (30.95%), respectively. When using 2.2 M pyruvate, in the first 6 h, the concentration of N-acetyl-D-neuraminic acid reaches 29.5 mM (which is about half of the maximal concentration), and the productivity of N-acetyl-D-neuraminic acid is 1.6 g/l/h, the concentration of the intermediate N-acetyl-D-mannosamine also increases gradually from 0 to 64 mM during the process	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-acetyl-D-neuraminic acid	-	Escherichia coli	N-acetyl-D-mannosamine + pyruvate	-	r

Synonyms

Synonyms	Comment	Organism
N-acetyl-d-neuraminic acid aldolase	-	Escherichia coli
Neu5Ac aldolase	-	Escherichia coli

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Release 2023.1 (January 2023)