Application | Comment | Organism |
---|---|---|
biotechnology | coupled bioconversion for preparation of N-acetyl-D-neuraminic acid using immobilized N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid lyase, a two-step enzymatic system involving immobilized both enzymes is used for the conversion of GlcNAc to NeuAc in a single reactor, optimum ratio is 3-6.25 U/ml of N-acetyl-D-glucosamine-2-epimerase and 12.5-25 U/ml of N-acetyl-D-neuraminic acid lyase | Sus scrofa |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
expression in Escherichia coli | Sus scrofa |
General Stability | Organism |
---|---|
Amberzyme oxirane resin-immobilized NAL can be used up to five reaction cycles without loss of activity or significant decrease of the conversion rate, the activity yield of immobilized NAL is approximately 10% of the free enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-neuraminic acid | Sus scrofa | - |
N-acetyl-D-mannosamine + pyruvate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli TG1 | - |
- |
- |
Sus scrofa | Q9BEC7 | - |
- |
Purification (Comment) | Organism |
---|---|
by FP-based affinity chromatography | Sus scrofa |
FPIDA-Co affinity support column chromatography | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cell density of Escherichia coli BL21 (DE3) carrying the pNA1 plasmid is 37 (OD600) after fermentation for 15 h, and the highest activity for N-acetyl-D-neuraminic acid lyase is 8.472 U/ml after fermentation for 9 h, enzyme activity is assayed in reaction solution (20 mM Neu5Ac in 50 mM potassium phosphate buffer, pH 7.5) | Sus scrofa |
0.0693 | - |
immobilized recombinant enzyme, the activity yield of immobilized enzyme is approximately 10% of the free enzyme | Sus scrofa |
2.45 | - |
crude extract, in 50 mM potassium phosphate buffer, pH 7.5, at 37°C | Escherichia coli |
36.26 | - |
after 14.8fold purification, in 50 mM potassium phosphate buffer, pH 7.5, at 37°C | Escherichia coli |
36.26 | - |
purified enzyme after expression in Escherichia coli cells, enzyme activity is assayed in reaction solution (20 mM N-acetyl-D-neuraminic acid in 50 mM potassium phosphate buffer, pH 7.5) | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-neuraminic acid | - |
Sus scrofa | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | - |
Escherichia coli | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | - |
Escherichia coli TG1 | N-acetyl-D-mannosamine + pyruvate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
N-acetyl-D-neuraminic acid lyase | - |
Escherichia coli |
N-acetyl-D-neuraminic acid lyase | - |
Sus scrofa |
NAL | - |
Escherichia coli |
NAL | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |