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Literature summary for 4.1.3.3 extracted from

  • Williams, G.J.; Woodhall, T.; Nelson, A.; Berry, A.
    Structure-guided saturation mutagenesis of N-acetylneuraminic acid lyase for the synthesis of sialic acid mimetics (2005), Protein Eng. Des. Sel., 18, 239-246.
    View publication on PubMed
Search for more literature for 4.1.3.3

Protein Variants

Protein Variants Comment Organism
D191P ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 1.4fold higher than the wild-type ratio Escherichia coli
E192N ratio of turnover-number to KM-value for N-acetyl-D-neuraminic acid is 13.4fold lower than the wild-type ratio, ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 48.7fold higher than the wild-type ratio Escherichia coli
E192S ratio of turnover-number to KM-value for N-acetyl-D-neuraminic acid is 3.9fold lower than the wild-type ratio, ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 32.2fold higher than the wild-type ratio Escherichia coli
E192V ratio of turnover-number to KM-value for N-acetyl-D-neuraminic acid is 17.9fold lower than the wild-type ratio, ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 39.3fold higher than the wild-type ratio Escherichia coli
S208G ratio of turnover-number to KM-value for N-acetyl-D-neuraminic acid is 2.5fold lower than the wild-type ratio, ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 1.5fold higher than the wild-type ratio Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192V Escherichia coli
0.39
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192N Escherichia coli
0.5
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192S Escherichia coli
2.54
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme S208G Escherichia coli
4.4
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, wild-type enzyme Escherichia coli
5.7
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme D191P Escherichia coli
10.6
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, wild-type enzyme Escherichia coli
10.7
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme S208G Escherichia coli
17
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192S Escherichia coli
34
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192V Escherichia coli
38
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192N Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
 Escherichia coli ?
-
 ?
N-acetyl-D-neuraminic acid
-
 Escherichia coli N-acetyl-D-mannosamine + pyruvate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme S208G Escherichia coli
0.91
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme D191P Escherichia coli
1.22
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, wild-type enzyme Escherichia coli
1.58
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192V Escherichia coli
1.85
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192S Escherichia coli
1.97
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192V Escherichia coli
2.18
-
 (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192N Escherichia coli
2.77
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192N Escherichia coli
4.15
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme S208G Escherichia coli
4.25
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192S Escherichia coli
4.3
-
 N-acetyl-D-neuraminic acid pH 7.5, 25°C, wild-type enzyme Escherichia coli