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Literature summary for 4.1.3.3 extracted from

  • Schauer, R.; Wember, M.
    Isolation and characterization of sialate lyase from pig kidney (1996), Biol. Chem. Hoppe-Seyler, 377, 293-299.
    View publication on PubMed
Search for more literature for 4.1.3.3

Inhibitors

Inhibitors Comment Organism Structure
5,5-dithiobis(2-nitrobenzoic acid) 50% residual activity at 0.3 mg/ml Sus scrofa
5-Diazonium-1-H-tetrazole 20% residual activity at 5 mM Sus scrofa
CN- 50% residual activity at 20 mM Sus scrofa
diethyldicarbonate 50% residual activity at 0.1% Sus scrofa
NaBH4
-
 Sus scrofa
o-phenanthroline 40% residual activity at 0.1 mM Sus scrofa
p-chloromercuribenzoate 70% residual activity at 0.1 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
 N-acetylneuraminic acid
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Sus scrofa
61000
-
 SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Oxidation Stability

Oxidation Stability Organism
sensitive to illumination in presence of Rose Bengal Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: N-acetyl-4-O-acetylneuraminic acid and 2-deoxy-2,3-didehydro-N-acetylneuraminic acid Sus scrofa ?
-
 ?
N-Acetyl-9-O-acetylneuraminic acid
-
 Sus scrofa ?
-
 ?
N-Acetylneuraminic acid
-
 Sus scrofa N-Acetyl-D-mannosamine + pyruvate
-
 ?
N-Glycolylneuraminic acid
-
 Sus scrofa ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Sus scrofa