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Literature summary for 4.1.3.27 extracted from

  • Ziebart, K.T.; Toney, M.D.
    Nucleophile specificity in anthranilate synthase, aminodeoxychorismate synthase, isochorismate synthase, and salicylate synthase (2010), Biochemistry, 49, 2851-2859.
    View publication on PubMed
Search for more literature for 4.1.3.27

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant overexpressed in Escherichia coli cells (strain CB694) harboring the pSTC25 plasmid Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
Q147K does not exhibit anthranilate synthase activity. Using (NH4)2SO4, 29% of the wild-type activity is restored to the mutant, with anthranilate representing 97% of all products. Employs water as a nucleophile to a small extent Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 L-glutamine wild-type, at 25°C, in 100 mM phosphate buffer, pH 7.0, with 20 mM L-glutamine and 5 mM MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium
3.6
-
 L-glutamine mutant Q147K, at 25°C, in 100 mM phosphate buffer, pH 7.0, with 20 mM L-glutamine and 5 mM MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant purified by hydroxyapatite chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
-80°C, 20 mM KH2PO4 buffer, 50 mM KCl, 1 mM DTT, pH 7.5 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-glutamine
-
 Salmonella enterica subsp. enterica serovar Typhimurium anthranilate + pyruvate + L-glutamate
-
 ?
chorismate + NH3
-
 Salmonella enterica subsp. enterica serovar Typhimurium anthranilate + pyruvate + H2O
-
 ?
additional information does not possess chorismate mutase promiscuous activity Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Synonyms

Synonyms Comment Organism
anthranilate synthase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
138
-
 L-glutamine wild-type, at 25°C, in 100 mM phosphate buffer, pH 7.0, with 20 mM L-glutamine and 5 mM MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium
152
-
 L-glutamine mutant Q147K, at 25°C, in 100 mM phosphate buffer, pH 7.0, with 20 mM L-glutamine and 5 mM MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium