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Literature summary for 4.1.3.24 extracted from
- Malyl-CoA lyase from Methylobacterium extorquens AM1 (1990), Methods Enzymol., 188, 379-391.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
acetyl-CoA | - |
Methylorubrum extorquens |  |
| 0.066 | - |
(3S)-3-carboxy-3-hydroxypropanoyl-CoA | - |
Methylorubrum extorquens | |
| 1.7 | - |
glyoxylate | - |
Methylorubrum extorquens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Methylorubrum extorquens | |
| Mg2+ | Km: 1.2 mM | Methylorubrum extorquens | |
| Mg2+ | divalent cation required, Mg2+ or Co2+ is most effective | Methylorubrum extorquens | |
| Mn2+ | divalent cation required, 88% of the activation with Mg2+ and Co2+ | Methylorubrum extorquens | |
| Zn2+ | divalent cation required, 41% of the activation with Mg2+ and Co2+ | Methylorubrum extorquens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
meniscus depletion method | Methylorubrum extorquens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Methylorubrum extorquens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylorubrum extorquens | - |
strain AM1, i.e. Pseudomonas sp. strain AM1, a mutant of strain AM1 lacking the enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methylorubrum extorquens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.1 | - |
- |
Methylorubrum extorquens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, stable for several months | Methylorubrum extorquens |
| 0-4°C, 2-3 weeks, stable | Methylorubrum extorquens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-3-carboxy-3-hydroxypropanoyl-CoA | - |
Methylorubrum extorquens | acetyl-CoA + glyoxylate | - |
r | |
| acetyl-CoA + glyoxylate | - |
Methylorubrum extorquens | (3S)-3-carboxy-3-hydroxypropanoyl-CoA | - |
r | |
| additional information | - |
Methylorubrum extorquens | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
irreversible loss of activity | Methylorubrum extorquens |
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