BRENDA - Enzyme Database show
show all sequences of 4.1.3.22

A comprehensive survey on isoleucine biosynthesis pathways in seven epidemic Leptospira interrogans reference strains of China

Zou, Y.; Guo, X.; Picardeau, M.; Xu, H.; Zhao, G.; FEMS Microbiol. Lett. 269, 90-96 (2007)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leptospira biflexa
A0FDJ2
fragment; serovar Monteralerio
-
Leptospira biflexa
A0FDJ4
fragment; serovar Patoc I
-
Leptospira interrogans
A0FDH8
fragment; serovar Lai
-
Leptospira interrogans
A0FDI0
fragment; serovar Canicola
-
Leptospira interrogans
A0FDI2
fragment; serovar Pyrogenes
-
Leptospira interrogans
A0FDI4
fragment; serovar Autumnalis
-
Leptospira interrogans
A0FDI6
fragment; serovar Australis
-
Leptospira interrogans
A0FDI8
fragment; serovar Pomona
-
Leptospira interrogans
A0FDJ0
fragment; serovar Hebdomadis
-
Leptospira meyeri
A1Z0Z6
fragment; serovar Semaranga
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
33.14
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira biflexa
37.43
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
39.42
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira biflexa
44.35
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
51.29
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
53.68
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
64.75
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
76.46
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
80.92
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira meyeri
82.36
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetate + pyruvate
-
679907
Leptospira interrogans
citramalate
-
-
-
?
acetate + pyruvate
-
679907
Leptospira meyeri
citramalate
-
-
-
?
acetate + pyruvate
-
679907
Leptospira biflexa
citramalate
-
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
33.14
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira biflexa
37.43
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
39.42
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira biflexa
44.35
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
51.29
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
53.68
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
64.75
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
76.46
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
80.92
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira meyeri
82.36
-
in TES buffer (0.1 M, pH 7.5), at 37C
Leptospira interrogans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetate + pyruvate
-
679907
Leptospira interrogans
citramalate
-
-
-
?
acetate + pyruvate
-
679907
Leptospira meyeri
citramalate
-
-
-
?
acetate + pyruvate
-
679907
Leptospira biflexa
citramalate
-
-
-
?
Other publictions for EC 4.1.3.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748351
Wu
Synthesis of citramalic acid ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Ind. Microbiol. Biotechnol.
44
1483-1490
2017
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1
1
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748589
Parimi
Eliminating acetate formation ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Microb. Cell Fact.
16
114
2017
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1
1
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1
1
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692849
Risso
Elucidation of an alternate is ...
Geobacter sulfurreducens, Geobacter sulfurreducens ATCC 51573 / DSM 12127
J. Bacteriol.
190
2266-2274
2008
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3
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679907
Zou
A comprehensive survey on isol ...
Leptospira biflexa, Leptospira interrogans, Leptospira meyeri
FEMS Microbiol. Lett.
269
90-96
2007
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15
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680494
Drevland
Enzymology and evolution of th ...
Methanocaldococcus jannaschii
J. Bacteriol.
189
4391-4400
2007
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6
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666279
Filatova
-
A study of the mechanism of ac ...
Rhodobacter sphaeroides
Microbiology
74
270-278
2005
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33256
Dimroth
Isolation and function of the ...
Clostridium tetanomorphum
Eur. J. Biochem.
80
469-477
1977
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1
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33257
Dimroth
Structure of the prosthetic gr ...
Clostridium tetanomorphum
FEBS Lett.
76
280-283
1977
1
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33255
Buckel
The enzyme complex citramalate ...
Clostridium tetanomorphum
Eur. J. Biochem.
64
255-262
1976
1
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2
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33258
Buckel
-
Substrate stereochemistry of t ...
Clostridium tetanomorphum
Biochem. Soc. Trans.
3
924-926
1975
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33254
Barker
-
Citramalate pyruvate lyase ...
Clostridium tetanomorphum
Methods Enzymol.
13
344-346
1969
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4
1
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2
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33253
Barker
Citramalate lyase of Clostridi ...
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920, no activity in Clostridium kluyveri, no activity in Escherichia coli, no activity in Pseudomonas sp., no activity in Saccharomyces cerevisiae
Arch. Mikrobiol.
59
4-12
1967
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4
1
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1
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17
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4
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2
1
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4
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