Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme contains acetyl thioester residues which play the central role in the catalytic mechanism | Clostridium tetanomorphum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | - |
Clostridium tetanomorphum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
520000 | 580000 | gel filtration, sucrose density gradient centrifugation | Clostridium tetanomorphum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetanomorphum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium tetanomorphum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Clostridium tetanomorphum |
Storage Stability | Organism |
---|---|
-20°C, enzyme concentration 10 mg/ml, 0.1 M phoshate, pH 7.4, 1.0 mM MgCl2, stable for at least 6 months | Clostridium tetanomorphum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetate + pyruvate | - |
Clostridium tetanomorphum | (+)-citramalate | - |
? | |
citramalate | (+)-citramalate | Clostridium tetanomorphum | acetate + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | 6 * 53000-56000, alpha, + 6 * 33000-36000, beta, + 6 * 10000-12000, gamma, SDS-PAGE | Clostridium tetanomorphum |