Data extracted from this reference:
Activating Compound
additional information
the enzyme contains acetyl thioester residues which play the central role in the catalytic mechanism
Clostridium tetanomorphum
Inhibitors
iodoacetate
Clostridium tetanomorphum
Molecular Weight [Da]
520000
580000
gel filtration, sucrose density gradient centrifugation
Clostridium tetanomorphum
Organism
Clostridium tetanomorphum
Purification (Commentary)
Clostridium tetanomorphum
Specific Activity [micromol/min/mg]
additional information
Clostridium tetanomorphum
Storage Stability
-20°C, enzyme concentration 10 mg/ml, 0.1 M phoshate, pH 7.4, 1.0 mM MgCl2, stable for at least 6 months
Clostridium tetanomorphum
Substrates and Products (Substrate)
acetate + pyruvate
33255
Clostridium tetanomorphum
(+)-citramalate
33255
Clostridium tetanomorphum
?
citramalate
(+)-citramalate
33255
Clostridium tetanomorphum
acetate + pyruvate
33255
Clostridium tetanomorphum
?
Subunits
oligomer
6 * 53000-56000, alpha, + 6 * 33000-36000, beta, + 6 * 10000-12000, gamma, SDS-PAGE
Clostridium tetanomorphum
Activating Compound (protein specific)
additional information
the enzyme contains acetyl thioester residues which play the central role in the catalytic mechanism
Clostridium tetanomorphum
Inhibitors (protein specific)
iodoacetate
Clostridium tetanomorphum
Molecular Weight [Da] (protein specific)
520000
580000
gel filtration, sucrose density gradient centrifugation
Clostridium tetanomorphum
Purification (Commentary) (protein specific)
Clostridium tetanomorphum
Specific Activity [micromol/min/mg] (protein specific)
additional information
Clostridium tetanomorphum
Storage Stability (protein specific)
-20°C, enzyme concentration 10 mg/ml, 0.1 M phoshate, pH 7.4, 1.0 mM MgCl2, stable for at least 6 months
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
acetate + pyruvate
33255
Clostridium tetanomorphum
(+)-citramalate
33255
Clostridium tetanomorphum
?
citramalate
(+)-citramalate
33255
Clostridium tetanomorphum
acetate + pyruvate
33255
Clostridium tetanomorphum
?
Subunits (protein specific)
oligomer
6 * 53000-56000, alpha, + 6 * 33000-36000, beta, + 6 * 10000-12000, gamma, SDS-PAGE
Clostridium tetanomorphum
Other publictions for EC 4.1.3.22
748351
Wu
Synthesis of citramalic acid ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Ind. Microbiol. Biotechnol.
44
1483-1490
2017
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1
1
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7
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748589
Parimi
Eliminating acetate formation ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Microb. Cell Fact.
16
114
2017
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1
1
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5
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2
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1
1
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692849
Risso
Elucidation of an alternate is ...
Geobacter sulfurreducens, Geobacter sulfurreducens ATCC 51573 / DSM 12127
J. Bacteriol.
190
2266-2274
2008
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3
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5
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4
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679907
Zou
A comprehensive survey on isol ...
Leptospira interrogans, Leptospira biflexa, Leptospira meyeri
FEMS Microbiol. Lett.
269
90-96
2007
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16
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10
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680494
Drevland
Enzymology and evolution of th ...
Methanocaldococcus jannaschii
J. Bacteriol.
189
4391-4400
2007
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6
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666279
Filatova
-
A study of the mechanism of ac ...
Rhodobacter sphaeroides
Microbiology
74
270-278
2005
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33256
Dimroth
Isolation and function of the ...
Clostridium tetanomorphum
Eur. J. Biochem.
80
469-477
1977
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1
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1
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33257
Dimroth
Structure of the prosthetic gr ...
Clostridium tetanomorphum
FEBS Lett.
76
280-283
1977
1
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1
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33255
Buckel
The enzyme complex citramalate ...
Clostridium tetanomorphum
Eur. J. Biochem.
64
255-262
1976
1
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1
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6
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1
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2
1
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33258
Buckel
-
Substrate stereochemistry of t ...
Clostridium tetanomorphum
Biochem. Soc. Trans.
3
924-926
1975
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33254
Barker
-
Citramalate pyruvate lyase ...
Clostridium tetanomorphum
Methods Enzymol.
13
344-346
1969
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1
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4
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33253
Barker
Citramalate lyase of Clostridi ...
Clostridium tetanomorphum, no activity in Saccharomyces cerevisiae, no activity in Clostridium kluyveri, no activity in Escherichia coli, no activity in Pseudomonas sp., Clostridium tetanomorphum H1 / ATCC 15920
Arch. Mikrobiol.
59
4-12
1967
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4
1
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1
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18
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4
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2
1
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4
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1
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1
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4
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2
1
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