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Literature summary for 4.1.3.16 extracted from

  • Huang, A.; Baker, E.; Loomes, K.
    Use of a novel microtitration protocol to obtain diffraction-quality crystals of 4-hydroxy-2-oxoglutarate aldolase from Bos taurus (2014), Acta Crystallogr. Sect. F, 70, 1546-1549 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.16

Crystallization (Commentary)

Crystallization (Comment) Organism
hamging drop vapor diffusion method, using 100 mM HEPES-NaOH pH 7.0, 200 mM Na2SO4, 22.5% (w/v) PEG 3350, and 20% (v/v) glycerol or 100 mM Tris-HCl pH 8.0 and 1.8 M ammonium sulfate Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-Hydroxy-2-oxoglutarate Bos taurus
-
 Pyruvate + glyoxylate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus Q0P5I5
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography, Sepharose Q column chromatography, and Superdex 200 gel filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Hydroxy-2-oxoglutarate
-
 Bos taurus Pyruvate + glyoxylate
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Bos taurus
? x * 32553, electrospray ionization mass spectrometry Bos taurus

Synonyms

Synonyms Comment Organism
hOGA
-
 Bos taurus