Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.1 extracted from

  • Meijer, S.; Otero, J.; Olivares, R.; Andersen, M.R.; Olsson, L.; Nielsen, J.
    Overexpression of isocitrate lyase-glyoxylate bypass influence on metabolism in Aspergillus niger (2009), Metab. Eng., 11, 107-116.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into vector pCR2.1-AfpyrG and overexpressed in the pyrA-strain AB4.1 Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger N402
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
Aspergillus niger succinate + glyoxylate
-
?
isocitrate
-
Aspergillus niger N402 succinate + glyoxylate
-
?

Synonyms

Synonyms Comment Organism
ICL
-
Aspergillus niger
isocitrate lyase
-
Aspergillus niger

General Information

General Information Comment Organism
metabolism ICL overexpression and malonate addition has a significant impact on metabolism and on organic acid production profiles. Increased ICL expression does not result in an increased glyoxylate bypass flux, there is a global response with respect to gene expression, leading to an increased flux through the oxidative part of the TCA cycle. Instead of an increased production of succinate and malate, a major increase in fumarate production is observed. In the strain with overexpression of ICL the organic acid production shifts from fumarate towards malate production when malonate is added to the cultivation medium Aspergillus niger