Literature summary for 4.1.3.1 extracted from

Klinke, S.; Dauner, M.; Scott, G.; Kessler, B.; Witholt, B.
Inactivation of isocitrate lyase leads to increased production of medium-chain-length poly(3-hydroxyalkanoates) in Pseudomonas putida (2000), Appl. Environ. Microbiol., 66, 909-913.

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Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isocitrate	Pseudomonas putida	rate-limiting enzyme of the citric acid cycle	succinate + glyoxylate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	wild-type: KT2442, mutant: KT217, poly(3-hydroxyalkanoate)-overproducing mutant in which aceA gene is knocked out	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.002	-	knock-out mutant enzyme after growth on gluconate, crude extract	Pseudomonas putida
0.003	-	knock-out mutant enzyme after growth on gluconate + heptanoate, crude extract	Pseudomonas putida
0.043	-	wild-type enzyme after growth on gluconate, crude extract	Pseudomonas putida
0.321	-	wild-type enzyme after growth on gluconate + heptanoate, crude extract	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isocitrate	-	Pseudomonas putida	succinate + glyoxylate	-	?
isocitrate	rate-limiting enzyme of the citric acid cycle	Pseudomonas putida	succinate + glyoxylate	-	?

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Release 2023.1 (January 2023)