Activating Compound | Comment | Organism | Structure |
---|---|---|---|
calcium thiamine diphosphate | - |
Lactiplantibacillus plantarum | |
magnesium thiamine diphosphate | - |
Lactiplantibacillus plantarum | |
manganese thiamine diphosphate | - |
Lactiplantibacillus plantarum |
Cloned (Comment) | Organism |
---|---|
into the vector pET21a+ for expression in Escherichia coli BL21DE3 cells | Lactiplantibacillus plantarum |
into the vectors pET21a+ and pET28b+ for expression in Escherichia coli BL21DE3 cells | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
D-fructose 6-phosphate | apparent Km value, Mn2+ | Lactiplantibacillus plantarum | |
0.032 | - |
D-fructofuranose 6-phosphate | apparent Km value, Mg2+ | Lactiplantibacillus plantarum | |
0.16 | - |
D-fructose 6-phosphate | apparent Km value, Ca2+ | Lactiplantibacillus plantarum | |
2.9 | - |
phosphate | D-fructose 6-phosphate and phosphate | Lactiplantibacillus plantarum | |
3.6 | - |
D-xylulose 5-phosphate | xylulose 5-phosphate and phosphate | Lactiplantibacillus plantarum | |
7.5 | - |
phosphate | xylulose 5-phosphate and phosphate | Lactiplantibacillus plantarum | |
24 | - |
D-fructose 6-phosphate | D-fructose 6-phosphate and phosphate | Lactiplantibacillus plantarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Lactiplantibacillus plantarum | |
Mg2+ | - |
Lactiplantibacillus plantarum | |
Mn2+ | - |
Lactiplantibacillus plantarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
SDS-PAGE | Lactiplantibacillus plantarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate + phosphate | Lactiplantibacillus plantarum | - |
? | - |
? | |
D-Xylulose 5-phosphate + phosphate | Lactiplantibacillus plantarum | - |
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | Q88S87 | - |
- |
Lactiplantibacillus plantarum | Q88U67 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant phosphoketolase-2 is purified using Ni-NTA resin, a Biogel P6 and a Sephacryl-300 column, in a second purification approach a DEAE-Sepharose and a Sephadex-300 column are used | Lactiplantibacillus plantarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.052 | - |
purification step crude extract, histidine tail phosphoketolase-2 | Lactiplantibacillus plantarum |
0.2 | - |
purification step Biogel P6 column, histidine tail phosphoketolase-2 | Lactiplantibacillus plantarum |
0.61 | - |
purification step Sephacryl-300 column, histidine tail phosphoketolase-2 | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate + phosphate | - |
Lactiplantibacillus plantarum | ? | - |
? | |
D-Xylulose 5-phosphate + phosphate | - |
Lactiplantibacillus plantarum | Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphoketolase-1 | - |
Lactiplantibacillus plantarum |
phosphoketolase-2 | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
enzyme assay | Lactiplantibacillus plantarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Lactiplantibacillus plantarum |