BRENDA - Enzyme Database
show all sequences of 4.1.2.8

Tryptophan synthase, an allosteric molecular factory

Barends, T.R.; Dunn, M.F.; Schlichting, I.; Curr. Opin. Chem. Biol. 12, 593-600 (2008)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Engineering
Amino acid exchange
Commentary
Organism
Q114N
betaGln114Asn mutation, which shortens the betaGln114 side chain connecting the betaL3 loop with the rest of the protein, resulted in two fractions of enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
Y110V
betaThr110Val mutation, which replaces a hydrogen bonding partner to the substrate carboxylate with a methyl group severely decreased the ability to form the aminoacrylate external aldimine E(AA) and consequently severely impaired beta-reaction and alphabeta-reaction
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
indole-3-glycerol phosphate
alpha-site cleaves the C3-C30 bond of IGP
691963
Salmonella enterica subsp. enterica serovar Typhimurium
indole + D-glyceraldehyde 3-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
TrpS alphabeta-dimer
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Q114N
betaGln114Asn mutation, which shortens the betaGln114 side chain connecting the betaL3 loop with the rest of the protein, resulted in two fractions of enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
Y110V
betaThr110Val mutation, which replaces a hydrogen bonding partner to the substrate carboxylate with a methyl group severely decreased the ability to form the aminoacrylate external aldimine E(AA) and consequently severely impaired beta-reaction and alphabeta-reaction
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
indole-3-glycerol phosphate
alpha-site cleaves the C3-C30 bond of IGP
691963
Salmonella enterica subsp. enterica serovar Typhimurium
indole + D-glyceraldehyde 3-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
TrpS alphabeta-dimer
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 4.1.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748938
Jin
Cloning and characterization ...
Persicaria tinctoria
Plant Cell Rep.
35
2449-2459
2016
-
-
1
-
1
-
-
-
1
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
728444
Zhuang
Biosynthesis and emission of i ...
Oryza sativa
Phytochemistry
73
15-22
2012
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
1
1
1
728511
Ahmad
Benzoxazinoid metabolites regu ...
Zea mays
Plant Physiol.
157
317-327
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
691511
Kriechbaumer
Characterisation of the trypto ...
Zea mays
BMC Plant Biol.
8
44
2008
-
-
1
-
-
-
-
1
1
-
-
1
-
5
-
-
1
-
-
1
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
691784
Barends
Structure and mechanistic impl ...
Salmonella enterica subsp. enterica serovar Typhimurium
ChemBioChem
9
1024-1028
2008
-
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691963
Barends
Tryptophan synthase, an allost ...
Salmonella enterica subsp. enterica serovar Typhimurium
Curr. Opin. Chem. Biol.
12
593-600
2008
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693498
Zhang
Arabidopsis indole synthase, a ...
Arabidopsis thaliana
J. Integr. Plant Biol.
50
1070-1077
2008
-
-
1
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695226
Dunn
Tryptophan synthase: the worki ...
Salmonella enterica subsp. enterica serovar Typhimurium
Trends Biochem. Sci.
33
254-264
2008
-
-
-
-
2
-
7
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666055
Kulik
On the structural basis of the ...
Zea mays
J. Mol. Biol.
352
608-620
2005
-
-
1
1
-
-
-
-
1
-
-
1
-
1
-
-
1
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670626
Farag
(Z)-3-Hexenol induces defense ...
Zea mays
Planta
220
900-909
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670511
Frey
Transcriptional activation of ...
Zea mays
Phytochemistry
65
1047-1055
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670717
Frey
An herbivore elicitor activate ...
Zea mays
Proc. Natl. Acad. Sci. USA
97
14801-14806
2000
1
-
1
-
-
-
-
1
-
-
-
1
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
670713
Melanson
A deletion in an indole syntha ...
Zea mays
Proc. Natl. Acad. Sci. USA
94
13345-13350
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670884
Frey
Analysis of a chemical plant d ...
Zea mays
Science
277
696-699
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669170
Yanofsky
The enzymatic conversion of an ...
Escherichia coli
J. Biol. Chem.
223
171-184
1956
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-