BRENDA - Enzyme Database
show all sequences of 4.1.2.8

On the structural basis of the catalytic mechanism and the regulation of the alpha subunit of tryptophan synthase from Salmonella typhimurium and BX1 from maize, two evolutionarily related enzymes

Kulik, V.; Hartmann, E.; Weyand, M.; Frey, M.; Gierl, A.; Niks, D.; Dunn, M.F.; Schlichting, I.; J. Mol. Biol. 352, 608-620 (2005)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli
Zea mays
Crystallization (Commentary)
Crystallization (Commentary)
Organism
the crystal structure of BX1 suggusts that the faster catalytic rate of BX1 compared to the homologous alpha-subuni8t of tryptophan synthase EC 4.2.1.20, may due to a stabilzation of the active conformation, loop alphaL6 is closed and the catalytic glutamate, Glu134 is in the active conformation. There are two crystallographically independent molecules in the asymmetric unit of the rhombohedral BX1 crystal form
Zea mays
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Zea mays
9536
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
indole-3-glycerol phosphate
Zea mays
the indole is further converted to 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, a secondary plant metabolite. BX1 cleaves indole-3-glycerol phosphate significantly faster to indole and glyceraldehyde 3-phosphate than the homologous alpha-subunit of tryptophan synthase, EC 4.2.1.20
indole + glyceraldehyde 3-phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Zea mays
P42390
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Zea mays
Reaction
Reaction
Commentary
Organism
Reaction ID
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
the indole is further converted to 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, a secondary plant metabolite. BX1 cleaves indole-3-glycerol phosphate significantly faster to indole and glyceraldehyde 3-phosphate than the homologous alpha-subunit of tryptophan synthase, EC 4.2.1.20. In the primary metabolism, indole diffuses through the connecting tunnel to the beta-active site where it is condensed with serine to form tryptophan and water
Zea mays
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Zea mays
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
indole-3-glycerol phosphate
-
666055
Zea mays
indole + glyceraldehyde 3-phosphate
-
-
-
?
indole-3-glycerol phosphate
the indole is further converted to 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, a secondary plant metabolite. BX1 cleaves indole-3-glycerol phosphate significantly faster to indole and glyceraldehyde 3-phosphate than the homologous alpha-subunit of tryptophan synthase, EC 4.2.1.20
666055
Zea mays
indole + glyceraldehyde 3-phosphate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
BX1
a homolog of the alpha-subunit of bifunctional trypthophan synthase
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Zea mays
Crystallization (Commentary) (protein specific)
Crystallization
Organism
the crystal structure of BX1 suggusts that the faster catalytic rate of BX1 compared to the homologous alpha-subuni8t of tryptophan synthase EC 4.2.1.20, may due to a stabilzation of the active conformation, loop alphaL6 is closed and the catalytic glutamate, Glu134 is in the active conformation. There are two crystallographically independent molecules in the asymmetric unit of the rhombohedral BX1 crystal form
Zea mays
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Zea mays
9536
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
indole-3-glycerol phosphate
Zea mays
the indole is further converted to 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, a secondary plant metabolite. BX1 cleaves indole-3-glycerol phosphate significantly faster to indole and glyceraldehyde 3-phosphate than the homologous alpha-subunit of tryptophan synthase, EC 4.2.1.20
indole + glyceraldehyde 3-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Zea mays
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
indole-3-glycerol phosphate
-
666055
Zea mays
indole + glyceraldehyde 3-phosphate
-
-
-
?
indole-3-glycerol phosphate
the indole is further converted to 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, a secondary plant metabolite. BX1 cleaves indole-3-glycerol phosphate significantly faster to indole and glyceraldehyde 3-phosphate than the homologous alpha-subunit of tryptophan synthase, EC 4.2.1.20
666055
Zea mays
indole + glyceraldehyde 3-phosphate
-
-
-
?
Other publictions for EC 4.1.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748938
Jin
Cloning and characterization ...
Persicaria tinctoria
Plant Cell Rep.
35
2449-2459
2016
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1
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1
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1
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5
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1
1
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728444
Zhuang
Biosynthesis and emission of i ...
Oryza sativa
Phytochemistry
73
15-22
2012
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1
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5
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1
728511
Ahmad
Benzoxazinoid metabolites regu ...
Zea mays
Plant Physiol.
157
317-327
2011
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1
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691511
Kriechbaumer
Characterisation of the trypto ...
Zea mays
BMC Plant Biol.
8
44
2008
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1
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1
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5
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691784
Barends
Structure and mechanistic impl ...
Salmonella enterica subsp. enterica serovar Typhimurium
ChemBioChem
9
1024-1028
2008
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1
1
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691963
Barends
Tryptophan synthase, an allost ...
Salmonella enterica subsp. enterica serovar Typhimurium
Curr. Opin. Chem. Biol.
12
593-600
2008
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1
2
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1
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1
1
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693498
Zhang
Arabidopsis indole synthase, a ...
Arabidopsis thaliana
J. Integr. Plant Biol.
50
1070-1077
2008
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1
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1
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1
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4
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6
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1
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4
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1
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1
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1
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6
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1
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695226
Dunn
Tryptophan synthase: the worki ...
Salmonella enterica subsp. enterica serovar Typhimurium
Trends Biochem. Sci.
33
254-264
2008
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2
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7
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1
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7
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666055
Kulik
On the structural basis of the ...
Zea mays
J. Mol. Biol.
352
608-620
2005
-
-
1
1
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1
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1
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1
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670626
Farag
(Z)-3-Hexenol induces defense ...
Zea mays
Planta
220
900-909
2005
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1
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1
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1
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8
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670511
Frey
Transcriptional activation of ...
Zea mays
Phytochemistry
65
1047-1055
2004
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1
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3
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670717
Frey
An herbivore elicitor activate ...
Zea mays
Proc. Natl. Acad. Sci. USA
97
14801-14806
2000
1
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1
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4
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1
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2
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1
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670713
Melanson
A deletion in an indole syntha ...
Zea mays
Proc. Natl. Acad. Sci. USA
94
13345-13350
1997
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3
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670884
Frey
Analysis of a chemical plant d ...
Zea mays
Science
277
696-699
1997
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669170
Yanofsky
The enzymatic conversion of an ...
Escherichia coli
J. Biol. Chem.
223
171-184
1956
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