Application | Comment | Organism |
---|---|---|
additional information | studies on genetic manipulation of cell walls in plants | Nicotiana tabacum |
Cloned (Comment) | Organism |
---|---|
transgenic tobacco cv. XHFD 8 plants expressing various levels of bacterial HCHL, lignin deposition reduced, monolignol biosynthesis and vascular integrity impaired, reduction in stem toughness, cell wall esterified and soluble phenolics reduced, wall-bound accretion of vanillin and vanillic acid, intracellular accumulation of novel metabolites such as hydroxybenzoic and vanillic acid derivatives | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | impact on cell wall integrity and vascular integrity, biomechanical tissue properties, carbon allocation to phenolic pools, lignin formation studied, morphological changes of the plants described | Nicotiana tabacum | 5618 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl-CoA | Nicotiana tabacum | - |
4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
transgenic tobacco cv XHFD 8 expressing various levels of HCHL, the bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase gene of Pseudomonas fluorescens, transgenic lines 201 and 27 reveal high expression, line 13 shows intermediate expression, pBin 19 plants serve as controls | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young leaves from pre- and postflowering transgenic and control plants, cell wall components analysed by HPLC, plants expressing high levels of HCHL exhibit a reduced lignin deposition, monolignol biosynthesis and vascular integrity impaired | Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activity measured, quantification by integration of peaks with reference to vanillin, peak identity calculated by GC-MS and NMR | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl-CoA | - |
Nicotiana tabacum | 4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA | - |
? | |
p-coumaroyl-CoA | in transgenic plants the HCHL enzyme acts upon intermediates of the phenylpropanoid pathway | Nicotiana tabacum | 4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA | i.e. vanillin | ? |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxycinnamoyl-CoA hydratase/lyase | - |
Nicotiana tabacum |
HCHL | - |
Nicotiana tabacum |