Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.61 extracted from

  • Merali, Z.; Mayer, M.J.; Parker, M.L.; Michael, A.J.; Smith, A.C.; Waldron, K.W.
    Metabolic diversion of the phenylpropanoid pathway causes cell wall and morphological changes in transgenic tobacco stems (2007), Planta, 225, 1165-1178.
    View publication on PubMed

Application

Application Comment Organism
additional information studies on genetic manipulation of cell walls in plants Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment) Organism
transgenic tobacco cv. XHFD 8 plants expressing various levels of bacterial HCHL, lignin deposition reduced, monolignol biosynthesis and vascular integrity impaired, reduction in stem toughness, cell wall esterified and soluble phenolics reduced, wall-bound accretion of vanillin and vanillic acid, intracellular accumulation of novel metabolites such as hydroxybenzoic and vanillic acid derivatives Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall impact on cell wall integrity and vascular integrity, biomechanical tissue properties, carbon allocation to phenolic pools, lignin formation studied, morphological changes of the plants described Nicotiana tabacum 5618
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl-CoA Nicotiana tabacum
-
4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
transgenic tobacco cv XHFD 8 expressing various levels of HCHL, the bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase gene of Pseudomonas fluorescens, transgenic lines 201 and 27 reveal high expression, line 13 shows intermediate expression, pBin 19 plants serve as controls
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf young leaves from pre- and postflowering transgenic and control plants, cell wall components analysed by HPLC, plants expressing high levels of HCHL exhibit a reduced lignin deposition, monolignol biosynthesis and vascular integrity impaired Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme activity measured, quantification by integration of peaks with reference to vanillin, peak identity calculated by GC-MS and NMR Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
feruloyl-CoA
-
Nicotiana tabacum 4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA
-
?
p-coumaroyl-CoA in transgenic plants the HCHL enzyme acts upon intermediates of the phenylpropanoid pathway Nicotiana tabacum 4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA i.e. vanillin ?

Synonyms

Synonyms Comment Organism
4-hydroxycinnamoyl-CoA hydratase/lyase
-
Nicotiana tabacum
HCHL
-
Nicotiana tabacum