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Literature summary for 4.1.2.61 extracted from

  • Leonard, P.M.; Marshall, C.M.; Dodson, E.J.; Walton, N.J.; Grogan, G.
    Purification, crystallization and preliminary x-ray crystallographic analysis of hydroxycinnamoyl-coenzyme A hydratase-lyase (HCHL), a crotonase homologue active in phenylpropanoid metabolism (2004), Acta Crystallogr. Sect. D, D60, 2343-2345.
    View publication on PubMed
Search for more literature for 4.1.2.61

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Pseudomonas fluorescens

Crystallization (Commentary)

Crystallization (Comment) Organism
by the hanging-drop method of vapour diffusion using polyethylene glycol 20000 Da as the precipitant, 1.8 A resolution Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31007
-
 6 * 31007, crystallization, calculated from amino acid sequence Pseudomonas fluorescens
186000
-
 calculated from crystallization data Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-feruloyl-CoA + H2O Pseudomonas fluorescens
-
 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
trans-feruloyl-CoA + H2O Pseudomonas fluorescens AN103
-
 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens AN103
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from Escherichia coli Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-feruloyl-CoA + H2O
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
trans-feruloyl-CoA + H2O
-
 Pseudomonas fluorescens AN103 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 31007, crystallization, calculated from amino acid sequence Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
4-hydroxycinnamoyl-coenzyme A hydratase-lyase
-
 Pseudomonas fluorescens
FCHL
-
 Pseudomonas fluorescens
feruloyl-CoA hydratase-lyase
-
 Pseudomonas fluorescens
HCHL
-
 Pseudomonas fluorescens