Application | Comment | Organism |
---|---|---|
synthesis | biotechnological production of vanillin | Pseudomonas fluorescens |
Cloned (Comment) | Organism |
---|---|
gene encoding an enoyl-SCoA hydratase/lyase isolated, cloned and heterologously expressed in Escherichia coli JM109 | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
gel filtration | Pseudomonas fluorescens |
31000 | - |
alpha, alpha, 2 * 31000, SDS-PAGE, deduced from gene sequence | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA | Pseudomonas fluorescens | - |
vanillin + acetyl-CoA | - |
? | |
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA | Delftia acidovorans | - |
vanillin + acetyl-CoA | - |
? | |
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | Pseudomonas fluorescens | - |
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA | - |
? | |
trans-feruloyl-CoA + H2O | Pseudomonas fluorescens | - |
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens | - |
biovar V, strain AN103 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA | - |
Pseudomonas fluorescens | 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA | - |
? | |
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA | - |
Pseudomonas fluorescens | vanillin + acetyl-CoA | - |
? | |
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA | - |
Delftia acidovorans | vanillin + acetyl-CoA | - |
? | |
4-hydroxy-3-methoxy-phenyl-beta-hydroxypropionyl-CoA + H2O | - |
Pseudomonas fluorescens | vanillin + acetylCoA + feruloylCoA | - |
? | |
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | - |
Pseudomonas fluorescens | 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA | - |
? | |
feruloyl-CoA + H2O | - |
Pseudomonas fluorescens | vanillin + acetyl-CoA | - |
? | |
feruloyl-CoA + H2O | - |
Delftia acidovorans | vanillin + acetyl-CoA | - |
? | |
trans-feruloyl-CoA + H2O | - |
Pseudomonas fluorescens | 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha, alpha, 2 * 31000, SDS-PAGE, deduced from gene sequence | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxycinnamoyl-CoA hydratase/lyase | - |
Pseudomonas fluorescens |