Literature summary for 4.1.2.61 extracted from

Mitra, A.; Kitamura, Y.; Gasson, M.J.; Narbad, A.; Parr, A.J.; Pyne, J.; Rhodes, M.J.C.; Sewter, C.; Walton, N.J.
4-Hydroxycinnamoyl-CoA hydratase/lyase (HCHL)-an enzyme of phenylpropanoid chain cleavage from Pseudomonas (1999), Arch. Biochem. Biophys., 365, 10-16.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Pseudomonas fluorescens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.6	-	caffeoyl-CoA	-	Pseudomonas fluorescens
2.4	-	feruloyl-CoA	-	Pseudomonas fluorescens
5.2	-	4-coumaroyl-CoA	-	Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	alpha, alpha, 2 * 31000, SDS-PAGE, deduced from gene sequence	Pseudomonas fluorescens
63000	-	gel filtration	Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA	Pseudomonas fluorescens	-	4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA	-	?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA	Pseudomonas fluorescens AN103	-	4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA	-	?
trans-feruloyl-CoA + H2O	Pseudomonas fluorescens	-	4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA	-	?
trans-feruloyl-CoA + H2O	Pseudomonas fluorescens AN103	-	4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	-	-
Pseudomonas fluorescens AN103	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Mono-Q, Mono-P, Phenyl-Superose	Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.003	-	-	Pseudomonas fluorescens

Storage Stability

Storage Stability	Organism
-70°C, several months	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,4-dihydroxycinnamoyl-CoA + H2O	-	Pseudomonas fluorescens	3,4-dihydroxybenzaldehyde + acetyl-CoA	-	?
3,4-dihydroxycinnamoyl-CoA + H2O	-	Pseudomonas fluorescens AN103	3,4-dihydroxybenzaldehyde + acetyl-CoA	-	?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA	-	Pseudomonas fluorescens	4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA	-	?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA	-	Pseudomonas fluorescens AN103	4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA	-	?
4-hydroxycinnamoyl-CoA + H2O	-	Pseudomonas fluorescens	4-hydroxybenzaldehyde + acetyl-CoA	-	?
4-hydroxycinnamoyl-CoA + H2O	-	Pseudomonas fluorescens AN103	4-hydroxybenzaldehyde + acetyl-CoA	-	?
trans-feruloyl-CoA + H2O	-	Pseudomonas fluorescens	4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA	-	?
trans-feruloyl-CoA + H2O	-	Pseudomonas fluorescens AN103	4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA	-	?

Subunits

Subunits	Comment	Organism
dimer	alpha, alpha, 2 * 31000, SDS-PAGE, deduced from gene sequence	Pseudomonas fluorescens

Synonyms

Synonyms	Comment	Organism
4-hydroxycinnamoyl-CoA hydratase/lyase	enzyme has hydratase and lyase activity	Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.3	-	4-coumaroyl-CoA	at 30°C	Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	9.5	in Tris-HCl or borate buffer, activity declined to ca. 50% of its maximum at pH 6.5	Pseudomonas fluorescens

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Release 2023.1 (January 2023)