BRENDA - Enzyme Database show
show all sequences of 4.1.2.56

Novel benzene ring biosynthesis from C3 and C4 primary metabolites by two enzymes

Suzuki, H.; Ohnishi, Y.; Furusho, Y.; Sakuda, S.; Horinouchi, S.; J. Biol. Chem. 281, 36944-36951 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Streptomyces griseus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0056
-
L-aspartate 4-semialdehyde
pH 7.2, 30C
Streptomyces griseus
0.14
-
glycerone phosphate
pH 7.2, 30C
Streptomyces griseus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Streptomyces griseus
33000
-
10 * 33000, SDS-PAGE
Streptomyces griseus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerone phosphate + L-aspartate 4-semialdehyde
Streptomyces griseus
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
?
glycerone phosphate + L-aspartate 4-semialdehyde
Streptomyces griseus TK21
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces griseus
A0JC77
-
-
Streptomyces griseus TK21
A0JC77
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces griseus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerone phosphate + L-aspartate 4-semialdehyde
-
722650
Streptomyces griseus
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
glycerone phosphate + L-aspartate 4-semialdehyde
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
722650
Streptomyces griseus
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
glycerone phosphate + L-aspartate 4-semialdehyde
-
722650
Streptomyces griseus TK21
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
glycerone phosphate + L-aspartate 4-semialdehyde
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
722650
Streptomyces griseus TK21
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
Subunits
Subunits
Commentary
Organism
decamer
10 * 33000, SDS-PAGE
Streptomyces griseus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Streptomyces griseus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
stable below
Streptomyces griseus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
glycerone phosphate
pH 7.2, 30C
Streptomyces griseus
0.2
-
L-aspartate 4-semialdehyde
pH 7.2, 30C
Streptomyces griseus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces griseus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
10
1 h, 30C, stable
Streptomyces griseus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces griseus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0056
-
L-aspartate 4-semialdehyde
pH 7.2, 30C
Streptomyces griseus
0.14
-
glycerone phosphate
pH 7.2, 30C
Streptomyces griseus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Streptomyces griseus
33000
-
10 * 33000, SDS-PAGE
Streptomyces griseus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerone phosphate + L-aspartate 4-semialdehyde
Streptomyces griseus
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
?
glycerone phosphate + L-aspartate 4-semialdehyde
Streptomyces griseus TK21
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces griseus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerone phosphate + L-aspartate 4-semialdehyde
-
722650
Streptomyces griseus
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
glycerone phosphate + L-aspartate 4-semialdehyde
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
722650
Streptomyces griseus
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
glycerone phosphate + L-aspartate 4-semialdehyde
-
722650
Streptomyces griseus TK21
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
glycerone phosphate + L-aspartate 4-semialdehyde
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
722650
Streptomyces griseus TK21
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
decamer
10 * 33000, SDS-PAGE
Streptomyces griseus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Streptomyces griseus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
stable below
Streptomyces griseus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
glycerone phosphate
pH 7.2, 30C
Streptomyces griseus
0.2
-
L-aspartate 4-semialdehyde
pH 7.2, 30C
Streptomyces griseus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces griseus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
10
1 h, 30C, stable
Streptomyces griseus
General Information
General Information
Commentary
Organism
physiological function
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
Streptomyces griseus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
Streptomyces griseus
Other publictions for EC 4.1.2.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722650
Suzuki
Novel benzene ring biosynthesi ...
Streptomyces griseus, Streptomyces griseus TK21
J. Biol. Chem.
281
36944-36951
2006
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1
-
-
-
-
2
-
-
2
2
-
2
-
-
1
-
-
-
-
-
4
1
1
-
1
2
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
2
2
-
-
-
1
-
-
-
-
4
1
1
-
1
2
1
-
1
-
-
1
1
-
-
-