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Literature summary for 4.1.2.52 extracted from
- Comparison of two metal-dependent pyruvate aldolases related by convergent evolution: substrate specificity, kinetic mechanism, and substrate channeling (2010), Biochemistry, 49, 3774-3782.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the active site of HpaI is formed by approx. 30 residues from adjacent dimers and consists of an approx. 15 A deep bell-shaped cleft with an approx. 12 A wide mouth. This broad entrance to the active site is predominantly lined with noncharged residues and a few positively charged residues | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxobutanoate | competitive inhibition | Escherichia coli |  |
| 2-oxopentanoate | competitive inhibition | Escherichia coli | |
| 4-methyl-2-oxopentanoate | competitive inhibition | Escherichia coli | |
| glyoxylate | competitive inhibition | Escherichia coli | |
| pyruvate | competitive inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.6 | - |
pyruvate | steady-state kinetic parameter, pH 8.0 and 25°C | Escherichia coli | |
| 9.1 | - |
Succinic semialdehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 13.4 | - |
Butyraldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 32.9 | - |
propionaldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 33.3 | - |
glycolaldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 50.1 | - |
acetaldehyde | Km(app) with 2-oxobutanoate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 62.1 | - |
acetaldehyde | steady-state kinetic parameter, pH 8.0 and 25°C | Escherichia coli | |
| 62.9 | - |
acetaldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 73.8 | - |
Isobutyraldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 88.6 | - |
DL-glyceraldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + succinic semialdehyde | Escherichia coli | - |
4-hydroxy-2-oxo-1,7-heptanedioate | enzyme lacks stereospecific control producing racemic mixtures of its physiological substrate, 4-hydroxy-2-oxo-1,7-heptanedioate | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4-hydroxy-2-oxoheptanedioate = pyruvate + succinate semialdehyde | rapid equilibrium random order mechanism | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxobutanoate + acetaldehyde | - |
Escherichia coli | ? | - |
? | |
| pyruvate + acetaldehyde | - |
Escherichia coli | 4-hydroxy-2-oxopentanoate | enzyme lacks stereospecific control producing racemic mixtures of 4-hydroxy-2-oxopentanoate i.e. HOPA | ? | |
| pyruvate + butyraldehyde | - |
Escherichia coli | ? | - |
? | |
| pyruvate + DL-glyceraldehyde | - |
Escherichia coli | ? | - |
? | |
| pyruvate + glycolaldehyde | - |
Escherichia coli | ? | - |
? | |
| pyruvate + isobutyraldehyde | - |
Escherichia coli | ? | - |
? | |
| pyruvate + pentaldehyde | - |
Escherichia coli | ? | - |
? | |
| pyruvate + propionaldehyde | - |
Escherichia coli | ? | - |
? | |
| pyruvate + succinic semialdehyde | - |
Escherichia coli | 4-hydroxy-2-oxo-1,7-heptanedioate | enzyme lacks stereospecific control producing racemic mixtures of its physiological substrate, 4-hydroxy-2-oxo-1,7-heptanedioate | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HpaI | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.6 | - |
DL-glyceraldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 21.9 | - |
acetaldehyde | Kcat(app) with 2-oxobutanoate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 64.9 | - |
Isobutyraldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 132.5 | - |
Butyraldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 175.5 | - |
glycolaldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 203.8 | - |
Succinic semialdehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 205.4 | - |
acetaldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 219.5 | - |
pyruvate | steady-state kinetic parameter, with acetaldehyde as aldehyde donor, pH 8.0 and 25°C | Escherichia coli | |
| 358.4 | - |
propionaldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
glyoxylate | pH 8.0, 25°C | Escherichia coli | |
| 0.5 | - |
2-oxobutanoate | pH 8.0, 25°C | Escherichia coli | |
| 0.51 | - |
pyruvate | pH 8.0, 25°C | Escherichia coli | |
| 2.01 | - |
pyruvate | pH 8.0, 25°C | Escherichia coli | |
| 3.6 | - |
2-oxopentanoate | pH 8.0, 25°C | Escherichia coli | |
| 6.98 | - |
4-methyl-2-oxopentanoate | pH 8.0, 25°C | Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
DL-glyceraldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 0.4 | - |
acetaldehyde | Kcat/Km(app) with 2-oxobutanoate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 0.9 | - |
Isobutyraldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 3.3 | - |
acetaldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 5.27 | - |
glycolaldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 9.9 | - |
Butyraldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 10.9 | - |
propionaldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 20 | - |
pentaldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
| 22.2 | - |
Succinic semialdehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
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