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Literature summary for 4.1.2.5 extracted from

  • Dueckers, N.; Baer, K.; Simon, S.; Groeger, H.; Hummel, W.
    Threonine aldolases-screening, properties and applications in the synthesis of non-proteinogenic beta-hydroxy-alpha-amino acids (2010), Appl. Microbiol. Biotechnol., 88, 409-424.
    View publication on PubMed
Search for more literature for 4.1.2.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 L-allo-threonine pH and temperature not specified in the publication Escherichia coli
10
-
 L-threonine pH and temperature not specified in the publication Escherichia coli
14.6
-
 L-allo-threonine pH and temperature not specified in the publication Pseudomonas sp.
14.7
-
 L-threonine pH and temperature not specified in the publication Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-
Escherichia coli
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. NCIMB10558
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography Clostridium pasteurianum
DEAE-Toyopearl column chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity of 31 units/mg, using L-threonine as substrate, pH and temperature not specified in the publication Pseudomonas sp.
additional information
-
 the crude extract has a specific activity of 0.02 units/mg, the purified enzyme has a specific activity of 0.32 units/mg, pH and temperature not specified in the publication Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-allo-threonine
-
 Escherichia coli ?
-
 ?
L-allo-threonine
-
 Pseudomonas sp. ?
-
 ?
L-allo-threonine
-
 Pseudomonas sp. NCIMB10558 ?
-
 ?
L-threonine L-TA preferably accepts L-threonine as substrate Escherichia coli glycine + acetaldehyde
-
 ?
L-threonine L-TA preferably accepts L-threonine as substrate Pseudomonas aeruginosa glycine + acetaldehyde
-
 ?
L-threonine L-TA preferably accepts L-threonine as substrate Pseudomonas sp. glycine + acetaldehyde
-
 ?
L-threonine L-TA preferably accepts L-threonine as substrate Clostridium pasteurianum glycine + acetaldehyde
-
 ?
L-threonine L-TA preferably accepts L-threonine as substrate Pseudomonas sp. NCIMB10558 glycine + acetaldehyde
-
 ?

Synonyms

Synonyms Comment Organism
L-TA
-
 Escherichia coli
L-TA
-
 Pseudomonas aeruginosa
L-TA
-
 Pseudomonas sp.
L-TA
-
 Clostridium pasteurianum
L-threonine aldolase
-
 Escherichia coli
L-threonine aldolase
-
 Pseudomonas aeruginosa
L-threonine aldolase
-
 Pseudomonas sp.
L-threonine aldolase
-
 Clostridium pasteurianum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Escherichia coli
pyridoxal 5'-phosphate dependent on Pseudomonas aeruginosa
pyridoxal 5'-phosphate dependent on Pseudomonas sp.
pyridoxal 5'-phosphate dependent on Clostridium pasteurianum