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Literature summary for 4.1.2.47 extracted from
- Expression of hydroxynitrile lyase from Manihot esculenta in yeast and its application in (S)-mandelonitrile production using an immobilized enzyme reactor (2008), Biosci. Biotechnol. Biochem., 72, 1457-1463.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | (S)-mandelonitrile production on a commercial scale | Manihot esculenta |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| high expression in a multi-auxotrophic mutant of Saccharomyces cerevisiae | Manihot esculenta |
General Stability
| General Stability | Organism |
|---|---|
| the immobilized enzyme is successfully used for more than 20 repeated batches with no loss of conversion rate or enantioselectivity | Manihot esculenta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Manihot esculenta | P52705 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Manihot esculenta | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyanide + benzaldehyde | concentrations of benzaldehyde higher than 1 M result in decreased enantioselectivity due to nonenzymatic formation of racemic mandelonitrile due to an excess of benzaldehyde and cyanide | Manihot esculenta | (S)-mandelonitrile | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (S)-cyanohydrin producing hydroxynitrile lyase | - |
Manihot esculenta |
| Hydroxynitrile lyase | - |
Manihot esculenta |
| MeHNL | - |
Manihot esculenta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
immobilized enzyme | Manihot esculenta |
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