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Literature summary for 4.1.2.47 extracted from
- Properties of alpha-hydroxynitrile lyase from the petiole of cassava (Manihot esculenta Crantz) (1996), Arch. Biochem. Biophys., 334, 401-405.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-methyl-2-butanol | - |
Manihot esculenta |  |
| 2-Methylbutyraldehyde | - |
Manihot esculenta | |
| acetaldehyde | - |
Manihot esculenta | |
| acetone | - |
Manihot esculenta | |
| benzaldehyde | - |
Manihot esculenta | |
| butanol | - |
Manihot esculenta | |
| Chlorobutanol | - |
Manihot esculenta | |
| ethanol | - |
Manihot esculenta | |
| formaldehyde | - |
Manihot esculenta | |
| isobutanol | - |
Manihot esculenta | |
| Isobutyraldehyde | - |
Manihot esculenta | |
| methanol | - |
Manihot esculenta | |
| methyl ethyl ketone | - |
Manihot esculenta | |
| Octanol | - |
Manihot esculenta | |
| Pentanol | - |
Manihot esculenta | |
| phenol | - |
Manihot esculenta | |
| Propanol | - |
Manihot esculenta | |
| propionaldehyde | - |
Manihot esculenta | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
acetone cyanohydrin | - |
Manihot esculenta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25600 | - |
4 * 25600, SDS-PAGE | Manihot esculenta |
| 102000 | - |
gel filtration | Manihot esculenta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Manihot esculenta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Manihot esculenta |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| petiole | - |
Manihot esculenta | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47.5 | - |
- |
Manihot esculenta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-2-methylpropanenitrile | - |
Manihot esculenta | acetone + HCN | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 25600, SDS-PAGE | Manihot esculenta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Manihot esculenta |
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