Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Manihot esculenta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manihot esculenta | P52705 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Manihot esculenta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Manihot esculenta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanide + (2E)-but-2-enal | - |
Manihot esculenta | (3S,3E)-2-hydroxypent-3-enenitrile | 92% enantiomeric excess | ? | |
cyanide + (2E)-hex-2-enal | - |
Manihot esculenta | (2S,3E)-2-hydroxyhept-3-enenitrile | 97% enantiomeric excess | ? | |
cyanide + 1,3-benzodioxole-5-carbaldehyde | - |
Manihot esculenta | (2S)-1,3-benzodioxol-5-yl(hydroxy)acetonitrile | 86% enantiomeric excess | ? | |
cyanide + 2,2-dimethylpropanal | - |
Manihot esculenta | (2S)-2-hydroxy-3,3-dimethylbutanenitrile | 94% enantiomeric excess | ? | |
cyanide + 2-chlorobenzaldehyde | - |
Manihot esculenta | (2S)-(2-chlorophenyl)(hydroxy)acetonitrile | 92% enantiomeric excess | ? | |
cyanide + 2-methylpropanal | - |
Manihot esculenta | (2S)-2-hydroxy-3-methylbutanenitrile | 95% enantiomeric excess | ? | |
cyanide + 3,3-dimethylbutan-2-one | - |
Manihot esculenta | (2S)-2-hydroxy-2,3,3-trimethylbutanenitrile | 78% enantiomeric excess | ? | |
cyanide + 4-methoxybenzaldehyde | - |
Manihot esculenta | (2S)-hydroxy(4-methoxyphenyl)acetonitrile | 98% enantiomeric excess | ? | |
cyanide + 4-methylpentan-2-one | - |
Manihot esculenta | (2S)-2-hydroxy-2,4-dimethylpentanenitrile | 28% enantiomeric excess | ? | |
cyanide + benzaldehyde | - |
Manihot esculenta | (S)-mandelonitrile | 98% enantiomeric excess | ? | |
cyanide + butan-2-one | - |
Manihot esculenta | (2S)-2-hydroxy-2-methylbutanenitrile | 18% enantiomeric excess | ? | |
cyanide + cyclohexanecarbaldehyde | - |
Manihot esculenta | (2S)-cyclohexyl(hydroxy)acetonitrile | 92% enantiomeric excess | ? | |
cyanide + heptan-2-one | - |
Manihot esculenta | (2S)-2-hydroxy-2-methylheptanenitrile | 92% enantiomeric excess | ? | |
cyanide + hexan-2-one | - |
Manihot esculenta | (2S)-2-hydroxy-2-methylhexanenitrile | 80% enantiomeric excess | ? | |
cyanide + pentan-2-one | - |
Manihot esculenta | (2S)-2-hydroxy-2-methylpentanenitrile | 69% enantiomeric excess | ? | |
cyanide + pentanal | - |
Manihot esculenta | (2S)-2-hydroxyhexanenitrile | 91% enantiomeric excess | ? | |
cyanide + prop-2-enal | - |
Manihot esculenta | (2S)-2-hydroxybut-3-enenitrile | 47% enantiomeric excess | ? | |
cyanide + propanal | - |
Manihot esculenta | (2S)-2-hydroxybutanenitrile | 91% enantiomeric excess | ? | |
furan-3-carbaldehyde + HCN | - |
Manihot esculenta | (2S)-hydroxy(furan-3-yl)ethanenitrile | 92% enantiomeric excess | ? | |
additional information | the enzyme catalyzes enantioselective addition of HCN to aromatic, heteroaromatic and aliphatic aldehydes and ketones forming (S)-aldehyde cyanohydrins or (S)-ketone cyanohydrins | Manihot esculenta | ? | - |
? | |
thiophene-2-carbaldehyde + HCN | - |
Manihot esculenta | (2S)-hydroxy(thiophen-2-yl)ethanenitrile | 96% enantiomeric excess | ? | |
thiophene-3-carbaldehyde + HCN | - |
Manihot esculenta | (2S)-hydroxy(thiophen-3-yl)ethanenitrile | 98% enantiomeric excess | ? |
Synonyms | Comment | Organism |
---|---|---|
(S)-Hydroxynitrile lyase | - |
Manihot esculenta |
MeHNL | - |
Manihot esculenta |