Activating Compound | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 1 mM, 5% activation | Chlamydomonas reinhardtii |
Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is a powerful tool for the stereospecific synthesis of various beta-hydroxy amino acids in synthetic organic chemistry | Chlamydomonas reinhardtii |
Cloned (Comment) | Organism |
---|---|
overexpressed in recombinant Escherichia coli BL21 (DE3) cells | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 0.1 mM, 87% inhibition | Chlamydomonas reinhardtii | |
CoCl2 | 0.1 mM, 12% inhibition | Chlamydomonas reinhardtii | |
CuCl2 | 0.1 mM, complete inhibition | Chlamydomonas reinhardtii | |
EDTA | 1 mM, complete inhibition | Chlamydomonas reinhardtii | |
hydroxylamine | 1 mM, 95% inhibition | Chlamydomonas reinhardtii | |
iodoacetate | 1 mM, 11% inhibition | Chlamydomonas reinhardtii | |
MgCl2 | 0.1 mM, 24% inhibition | Chlamydomonas reinhardtii | |
NiCl2 | 0.1 mM, 16 % inhibition | Chlamydomonas reinhardtii | |
p-chloromercuribenzoate | 1 mM, 7% inhibition | Chlamydomonas reinhardtii | |
Sodium cyanide | 1 mM, 87% inhibition | Chlamydomonas reinhardtii | |
ZnCl2 | 0.1 mM, complete inhibition | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
D-threonine | pH 8.1, 50°C | Chlamydomonas reinhardtii | |
0.81 | - |
D-threo-phenylserine | pH 8.1, 50°C | Chlamydomonas reinhardtii | |
2.4 | - |
D-allo-threonine | pH 8.1, 50°C | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A0A1C9ZZ39 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.05 | - |
pH 8.1, 50°C | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allo-threonine | - |
Chlamydomonas reinhardtii | glycine + acetaldehyde | - |
r | |
D-threo-phenylserine | - |
Chlamydomonas reinhardtii | benzaldehyde + glycine | - |
r | |
D-threonine | - |
Chlamydomonas reinhardtii | glycine + acetaldehyde | - |
r | |
glycine + acetaldehyde | the enzyme catalyzes the synthesis of D- and D-allothreonine from a mixture of glycine and acetaldehyde. The diastereomer excess of D-threonine is 18% | Chlamydomonas reinhardtii | D-allothreonine | - |
r | |
glycine + acetaldehyde | the enzyme catalyzes the synthesis of D- and D-allothreonine from a mixture of glycine and acetaldehyde. The diastereomer excess of D-threonine is 18% | Chlamydomonas reinhardtii | D-threonine | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
cleavage of D-threonine | Chlamydomonas reinhardtii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, 90% of residual activity | Chlamydomonas reinhardtii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
D-threonine | pH 8.1, 50°C | Chlamydomonas reinhardtii | |
25 | - |
D-allo-threonine | pH 8.1, 50°C | Chlamydomonas reinhardtii | |
280 | - |
D-threo-phenylserine | pH 8.1, 50°C | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
cleavage of D-threonine, in 0.1 M HEPES-NaOH buffer | Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate-dependent enzyme | Chlamydomonas reinhardtii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
D-allo-threonine | pH 8.1, 50°C | Chlamydomonas reinhardtii | |
13 | - |
D-threonine | pH 8.1, 50°C | Chlamydomonas reinhardtii | |
350 | - |
D-threo-phenylserine | pH 8.1, 50°C | Chlamydomonas reinhardtii |