Cloned (Comment) | Organism |
---|---|
plasmid pKK-223-3 with T7 IPTG-inducible promoter and coding for the lacD2 gene product tagatose-1-6-biphosphate aldolase 2 transformed and overexpressed using the Escherichia coli JM109 strain | Streptococcus pyogenes |
Crystallization (Comment) | Organism |
---|---|
native enzyme and native enzyme in complex with dihydroxyacetone-P, by the hanging drop method. Native free TBP aldolase crystallized in the P212121 space group with four protomers in the asymmetric unit and diffracted to 1.87 A resolution. Folding of the polypeptide chain of TBP aldolase corresponds to a (alpha/beta)8 or TIM barrel fold. The liganded crystal structure is isomorphous with the crystal structure of the native aldolase and diffracted to 1.92 A resolution | Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by anion-exchange chromatography and gel filtration, to homogeneity | Streptococcus pyogenes |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
tagatose-1,6-biphosphate aldolase 2 | - |
Streptococcus pyogenes |
TBP aldolase | - |
Streptococcus pyogenes |