Application | Comment | Organism |
---|---|---|
synthesis | synthesis of statin intermediates. The recombinant enzyme can be potentially applied in the production of (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexose. The bioconversion process for production of (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexose from chloroacetaldehyde and acetaldehyde using the recombinant enzyme is studied and this process took 3 h for maximal conversion | Haemophilus influenzae |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
2-deoxy-D-ribose 5-phosphate | pH 7.5, temperature not specified in the publication | Haemophilus influenzae | |
24.77 | - |
2-deoxy-D-ribose | pH 7.5, temperature not specified in the publication | Haemophilus influenzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
SDS-PAGE | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | P44430 | - |
- |
Haemophilus influenzae DSM 11121 | P44430 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Haemophilus influenzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.1 | - |
pH 7.5, temperature not specified in the publication | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-ribose | - |
Haemophilus influenzae | D-glyceraldehyde + acetaldehyde | - |
r | |
2-deoxy-D-ribose | - |
Haemophilus influenzae DSM 11121 | D-glyceraldehyde + acetaldehyde | - |
r | |
2-deoxy-D-ribose 5-phosphate | - |
Haemophilus influenzae | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
r | |
2-deoxy-D-ribose 5-phosphate | - |
Haemophilus influenzae DSM 11121 | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
r | |
D-glyceraldehyde 3-phosphate + acetaldehyde | 2-deoxy-D-ribose 5-phosphate-producing activity is highest in the presence of 300 mM acetaldehyde | Haemophilus influenzae | 2-deoxy-D-ribose 5-phosphate | - |
r | |
D-glyceraldehyde 3-phosphate + acetaldehyde | 2-deoxy-D-ribose 5-phosphate-producing activity is highest in the presence of 300 mM acetaldehyde | Haemophilus influenzae DSM 11121 | 2-deoxy-D-ribose 5-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Haemophilus influenzae |
Synonyms | Comment | Organism |
---|---|---|
2-Deoxyribose-5-phosphate aldolase | - |
Haemophilus influenzae |
DERA | - |
Haemophilus influenzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Haemophilus influenzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | 10°C: about 40% of maximal activity, 50°C: about 50% of maximal activity | Haemophilus influenzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, no loss of activity | Haemophilus influenzae |
50 | - |
1 h, less than 20% loss of activity | Haemophilus influenzae |
60 | - |
1 h, complete loss of activity | Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Haemophilus influenzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 12 | pH 5.0: about 70% of maximal activity, pH 12.0: about 70% of maximal activity | Haemophilus influenzae |