Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is an interesting candidate for bio-catalysis of carbo-ligation reactions, which are central to synthetic chemistry | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
S238P | completely abolished catalytic activity in the retro-aldol reaction | Escherichia coli |
S238P/S239P | completely abolished catalytic activity in the retro-aldol reaction | Escherichia coli |
S239P | the mutant enzyme increases the enthalpy change at the transition state, relative to the wild-type enzyme, but concomitant loss in entropy causes an overall relative loss in the TS free energy change. This entropy loss, as measured by the temperature dependence of catalysed rates, is mirrored in both a drastic loss in dynamics of the enzyme, which contributes to phosphate binding, as well as an overall loss in anti-correlated motions distributed over the entire protein | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.096 | - |
2-deoxy-D-ribose 5-phosphate | pH 8.0, 30°C, wild-type enzyme | Escherichia coli | |
3 | - |
2-deoxy-D-ribose 5-phosphate | pH 8.0, 30°C, mutant enzyme S239P | Escherichia coli | |
9.6 | - |
2-deoxy-D-ribose 5-phosphate | pH 8.0, 30°C, mutant enzyme S238I/S239I | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6L0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-ribose 5-phosphate | - |
Escherichia coli | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
r | |
D-glyceraldehyde 3-phosphate + acetaldehyde | - |
Escherichia coli | 2-deoxy-D-ribose 5-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
2-Deoxyribose-5-phosphate aldolase | - |
Escherichia coli |
DERA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
2-deoxy-D-ribose 5-phosphate | pH 8.0, 30°C, mutant enzyme S238I/S239I | Escherichia coli | |
11 | - |
2-deoxy-D-ribose 5-phosphate | pH 8.0, 30°C, mutant enzyme S239P | Escherichia coli | |
15 | - |
2-deoxy-D-ribose 5-phosphate | pH 8.0, 30°C, wild-type enzyme | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the pentose phosphate pathway | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
2-deoxy-D-ribose 5-phosphate | pH 8.0, 30°C, mutant enzyme S238I/S239I | Escherichia coli | |
3.6 | - |
2-deoxy-D-ribose 5-phosphate | pH 8.0, 30°C, mutant enzyme S239P | Escherichia coli | |
130 | - |
2-deoxy-D-ribose 5-phosphate | pH 8.0, 30°C, wild-type enzyme | Escherichia coli |