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Literature summary for 4.1.2.4 extracted from
- Cloning and characterisation of a new 2-deoxy-D-ribose-5-phosphate aldolase from Rhodococcus erythropolis (2012), J. Biotechnol., 161, 174-180.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.84 | - |
2-deoxy-D-ribose 5-phosphate | pH 7.0, 25°C | Rhodococcus erythropolis |  |
| 418 | - |
2-deoxy-D-ribose | pH 7.0, 25°C | Rhodococcus erythropolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22900 | - |
2 * 22900, calculated, 2 * 2000, SDS-PAGE | Rhodococcus erythropolis |
| 44000 | - |
gel filtration | Rhodococcus erythropolis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| acetaldehyde | 300 mM, half-life 64 min | Rhodococcus erythropolis |
| dimethyl sulfoxide | 20%, about 90% residual activity | Rhodococcus erythropolis |
| Ethanol | 20%, about 50-60% residual activity | Rhodococcus erythropolis |
| Ethyl acetate | 20%, about 50-60% residual activity | Rhodococcus erythropolis |
| Methanol | 20%, about 50-60% residual activity | Rhodococcus erythropolis |
| methyl-tert-butylether | 20%, about 50-60% residual activity | Rhodococcus erythropolis |
| additional information | almost complete loss of lost activity in 20% tetrahydrofuran, acetonitrile and iso-propanol | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | JN665070 | - |
- |
| Rhodococcus erythropolis DSM 311 | JN665070 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-ribose | - |
Rhodococcus erythropolis | D-glyceraldehyde + acetaldehyde | - |
? | |
| 2-deoxy-D-ribose | - |
Rhodococcus erythropolis DSM 311 | D-glyceraldehyde + acetaldehyde | - |
? | |
| 2-deoxy-D-ribose 5-phosphate | - |
Rhodococcus erythropolis | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
? | |
| 2-deoxy-D-ribose 5-phosphate | - |
Rhodococcus erythropolis DSM 311 | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 22900, calculated, 2 * 2000, SDS-PAGE | Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Rhodococcus erythropolis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
85% of maximum activity | Rhodococcus erythropolis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
half-life 70 h | Rhodococcus erythropolis |
| 65 | - |
half-life 2 min | Rhodococcus erythropolis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.23 | - |
2-deoxy-D-ribose | pH 7.0, 25°C | Rhodococcus erythropolis | |
| 18 | - |
2-deoxy-D-ribose 5-phosphate | pH 7.0, 25°C | Rhodococcus erythropolis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rhodococcus erythropolis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
80% of maximum activity | Rhodococcus erythropolis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
half-life 20 days | Rhodococcus erythropolis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0029 | - |
2-deoxy-D-ribose | pH 7.0, 25°C | Rhodococcus erythropolis | |
| 3.78 | - |
2-deoxy-D-ribose 5-phosphate | pH 7.0, 25°C | Rhodococcus erythropolis | |
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