Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.4 extracted from

  • Lokanath, N.K.; Shiromizu, I.; Ohshima, N.; Nodake, Y.; Sugahara, M.; Yokoyama, S.; Kuramitsu, S.; Miyano, M.; Kunishima, N.
    Structure of aldolase from Thermus thermophilus HB8 showing the contribution of oligomeric state to thermostability (2004), Acta Crystallogr. Sect. D, 60, 1816-1823.
    View publication on PubMed
Search for more literature for 4.1.2.4

Crystallization (Commentary)

Crystallization (Comment) Organism
microbatch method, crystal structure of the enzyme and its complex with 2-deoxy-D-ribose 5-phosphate Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 2-deoxy-D-ribose 5-phosphate pH 7.4, 35°C Thermus thermophilus
0.075
-
 2-deoxy-D-ribose 5-phosphate pH 7.4, 25°C Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
 HB8
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 HB8
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose 5-phosphate
-
 Thermus thermophilus D-glyceraldehyde 3-phosphate + acetaldehyde
-
 ?
2-deoxy-D-ribose 5-phosphate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 D-glyceraldehyde 3-phosphate + acetaldehyde
-
 ?

Subunits

Subunits Comment Organism
tetramer in the crystal and in solution Thermus thermophilus

Synonyms

Synonyms Comment Organism
TtDERA
-
 Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 2-deoxy-D-ribose 5-phosphate pH 7.4, 25°C Thermus thermophilus
11.4
-
 2-deoxy-D-ribose 5-phosphate pH 7.4, 35°C Thermus thermophilus