Cloned (Comment) | Organism |
---|---|
expressen in Escherichia coli | Aeropyrum pernix |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
2-deoxy-D-ribose 5-phosphate | 50°C, pH 6.5 | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
4 * 24000, SDS-PAGE | Aeropyrum pernix |
93000 | - |
gel-filtration | Aeropyrum pernix |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | stable up to 40% for 30 min at 50°C | Aeropyrum pernix |
Methanol | stable up to 50% for 30 min at 50°C | Aeropyrum pernix |
N,N-dimethylformamide | stable up to 50% for 30 min at 50°C | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9Y948 | K1 | - |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
50°C | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-ribose 5-phosphate | - |
Aeropyrum pernix | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 24000, SDS-PAGE | Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
no loss of activity after 60 min | Aeropyrum pernix |
100 | - |
no loss of activity after 10 min | Aeropyrum pernix |