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Literature summary for 4.1.2.4 extracted from

  • DeSantis, G.; Liu, J.; Clark, D.P.; Heine, A.; Wilson, I.A.; Wong, C.H.
    Structure-based mutagenesis approaches toward expanding the substrate specificity of D-2-deoxyribose-5-phosphate aldolase (2003), Bioorg. Med. Chem., 11, 43-52.
    View publication on PubMed
Search for more literature for 4.1.2.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
G205E decreased activity towards the natural substrate Escherichia coli
K172E decreased activity towards the natural substrate Escherichia coli
R207E altered substrate specificity Escherichia coli
S238D altered substrate specificity Escherichia coli
S239E decreased activity towards the natural substrate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.64
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, wild-type Escherichia coli
4.3
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S239E mutant Escherichia coli
33
-
 D-2-deoxyribose pH 7.5, 25°C, R207E mutant Escherichia coli
39
-
 D-2-deoxyribose pH 7.5, 25°C, S238D mutant Escherichia coli
57
-
 D-2-deoxyribose pH 7.5, 25°C, wild-type Escherichia coli
61
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S238D mutant Escherichia coli
63
-
 D-2-deoxyribose pH 7.5, 25°C, K172E mutant Escherichia coli
67
-
 D-2-deoxyribose pH 7.5, 25°C, S239E mutant Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-deoxy-D-ribose 5-phosphate Escherichia coli
-
 D-glyceraldehyde 3-phosphate + acetaldehyde
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose 5-phosphate
-
 Escherichia coli D-glyceraldehyde 3-phosphate + acetaldehyde
-
 r
3-azidopropanal + 2 acetaldehyde
-
 Escherichia coli (4R,6R)-6-(2-azidoethyl)-4-hydroxyoxan-2-one + 2 H+
-
 ?
D-2-deoxyribose good substrate for the S238D mutant, weak substrate for the wild-type enzyme Escherichia coli glyceraldehyde + acetaldehyde
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.022
-
 D-2-deoxyribose pH 7.5, 25°C, K172E mutant Escherichia coli
0.064
-
 D-2-deoxyribose pH 7.5, 25°C, R207E mutant Escherichia coli
0.107
-
 D-2-deoxyribose pH 7.5, 25°C, wild-type Escherichia coli
0.175
-
 D-2-deoxyribose pH 7.5, 25°C, S239E mutant Escherichia coli
0.21
-
 D-2-deoxyribose pH 7.5, 25°C, S238D mutant Escherichia coli
0.58
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S238D mutant Escherichia coli
6.08
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S238D mutant Escherichia coli
41
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S239E mutant Escherichia coli
68
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, wild-type Escherichia coli