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Literature summary for 4.1.2.38 extracted from

  • Gonzales, B.; Vicuna, R.
    Benzaldehyde lyase, a novel thiamine PPi-requiring enzyme, from Pseudomonas fluorescens biovar I (1989), J. Bacteriol., 171, 2401-2405.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.38

Inhibitors

Inhibitors Comment Organism Structure
2-Acetoxy-1,2-bis(4'-methoxyphenyl)-ethanone 0.2 mM: 50% inhibition Pseudomonas fluorescens
Desoxyanisoin 0.4 mM: 50% inhibition Pseudomonas fluorescens
desoxybenzoin 0.9 mM: 50% inhibition Pseudomonas fluorescens
additional information benzoylformic acid at 0.9 mM, pyruvic acid at 10 mM, acetoin at 10 mM, N-ethylmaleimide at 20 mM, dithiothreitol at 5 mM are no inhibitors Pseudomonas fluorescens
thiamine diphosphate more than 0.5 mM: inhibition, requirement for 0.01 mM Pseudomonas fluorescens
Urea 4 mM: inactivation Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 Benzoin 37°C, pH 8 Pseudomonas fluorescens
0.0325
-
 Anisoin 37°C, pH 8 Pseudomonas fluorescens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ less effective than Mg2+ or Mn2+ or Ca2+ Pseudomonas fluorescens
Ca2+ 1 mM, absolute requirement of a divalent cation, may be substituted by Mn2+ or Mg2+ Pseudomonas fluorescens
Cu2+ less effective than Mg2+ or Mn2+ or Ca2+ Pseudomonas fluorescens
Mg2+ 1 mM, absolute requirement for a divalent cation, may be substituted by Mn2+ or Ca2+ Pseudomonas fluorescens
Mn2+ 1 mM, absolute requirement for a divalent cation, may be substituted by Mg2+ or Ca2+ Pseudomonas fluorescens
additional information Co2+, Zn2+, Ni2+, Fe2+, Al2+ at 1 mM are ineffective. KCl, NaCl, NH4Cl at 1-160 mM: no influence Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, SDS-PAGE Pseudomonas fluorescens
80000
-
 gel filtration Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
 strain biovar I
-

Purification (Commentary)

Purification (Comment) Organism
strain biovar I, partial Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11690
-
-
 Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, stable for at least 2 months Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anisoin
-
 Pseudomonas fluorescens 4-methoxybenzaldehyde + 4-methoxybenzaldehyde
-
 ?
benzoin
-
 Pseudomonas fluorescens benzaldehyde + benzaldehyde
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 broad Pseudomonas fluorescens

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 no activity at pH 6 and below, maximal activity at pH 7.5-8.5 Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate absolute requirement, 0.01 mM optimal, more than 0.5 mM: inhibition Pseudomonas fluorescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 2-Acetoxy-1,2-bis(4'-methoxyphenyl)-ethanone 37°C, pH 8 Pseudomonas fluorescens
0.4
-
 Desoxyanisoin 37°C, pH 8 Pseudomonas fluorescens
0.9
-
 desoxybenzoin 37°C, pH 8 Pseudomonas fluorescens