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Literature summary for 4.1.2.33 extracted from

  • Prestwich, G.D.; Angelastro, M.; de Palma, A.; Perino, M.A.
    Fucosterol epoxide lyase of insects: synthesis of labeled substrates and development of a partition assay (1985), Anal. Biochem., 151, 315-326.
    View publication on PubMed
Search for more literature for 4.1.2.33

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10 mM: 103% of maximal activity, slight activation Manduca sexta
NADH 10 mM: 139% of maximal activity, activation Manduca sexta
NADP+ 10 mM: 109% of maximal activity, activation Manduca sexta
NADPH 10 mM: 106% of maximal activity, slight activation Manduca sexta
p-hydroxymercuribenzoate 1 mM: 102% of maximal activity, slight activation Manduca sexta

General Stability

General Stability Organism
no activity is lost in a single freeze-thaw cycle Manduca sexta

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM: 16% of maximal activity Manduca sexta
chymotrypsin 0.05 mg/ml: 57% of maximal activity Manduca sexta
D-glucose 200 mM: 42% of maximal activity Manduca sexta
EDTA 10 mM: 66% of maximal activity Manduca sexta
Fe2+ 10 mM: 18% of maximal activity Manduca sexta
Fe3+ 10 mM: 26% of maximal activity Manduca sexta
glutathione oxidized, 10 mM: 58% of maximal activity, reduced: 10 mM, 89% of maximal activity Manduca sexta
imidazole 10 mM: 99% of maximal activity Manduca sexta
K+ 10 mM: 71% of maximal activity Manduca sexta
Lubrol Px 1% (w/v): complete inhibition Manduca sexta
Mg2+ 10 mM: 75% of maximal activity Manduca sexta
N-ethylmaleimide 10 mM: 77% of maximal activity Manduca sexta
NAD+ 10 mM: 95% of maximal activity Manduca sexta
phenylmethylsulfonyl fluoride 10 mM: 63% of maximal activity Manduca sexta
Staphylococcus aureus V8 protease 0.05 mg/ml: 20% of maximal activity Manduca sexta
sucrose 200 mM: 73% of maximal activity Manduca sexta
Triton X-100 1% (w/v): 12% of maximal activity Manduca sexta
Zn2+ 10 mM: 9% of maximal activity Manduca sexta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 (24RS,28RS)-fucosterol epoxide 37°C Manduca sexta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(24R,241R)-fucosterol epoxide Manduca sexta
-
 desmosterol + acetaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Manduca sexta
-
 tobacco hornworm
-

Purification (Commentary)

Purification (Comment) Organism
larval tobacco hornworm, partial Manduca sexta

Source Tissue

Source Tissue Comment Organism Textmining
fat body
-
 Manduca sexta
-
midgut
-
 Manduca sexta
-

Storage Stability

Storage Stability Organism
-80°C, no activity is lost in a single freeze-cycle Manduca sexta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(24R,241R)-fucosterol epoxide
-
 Manduca sexta desmosterol + acetaldehyde
-
 ?
(24R,241R)-fucosterol epoxide best substrate Manduca sexta desmosterol + acetaldehyde demonstrated in crude extract ?
(24RS,28RS)-fucosterol epoxide
-
 Manduca sexta ?
-
 ?
(24S,28S)-fucosterol epoxide
-
 Manduca sexta ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Manduca sexta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
27
-
 broad range of activity Manduca sexta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Manduca sexta