Application | Comment | Organism |
---|---|---|
analysis | easy and sensitive procedure to determine sphingosine-1-phosphate lyase activity. The assay uses C17-sphinganine-1-phosphate as substrate and the aldehyde product, pentadecanal, is quantified as its pentafluorobenzyloxime derivative by GC/MS. Derivatization of pentadecanal is performed as a one-step reaction, and the oxime product is directly injected for GC/MS analysis without any further purification. Limit of detection is 281 fmol. The assay is linear with both protein concentration and incubation time up to 20 microg and 40 min, respectively | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Deoxypyridoxine phosphate | - |
Mus musculus | |
FTY720 | produces an 82% inhibition at equimolar concentration with C17-sphinganine-1-phosphate | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
C17-sphinganine-1-phosphate | pH 7.4, 37°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic fibroblast | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C17-sphinganine-1-phosphate | - |
Mus musculus | phosphoethanolamine + pentadecanal | - |
? | |
sphinganine 1-phosphate | - |
Mus musculus | phosphoethanolamine + palmitaldehyde | - |
? |