Literature summary for 4.1.2.25 extracted from

Garcon, A.; Levy, C.; Derrick, J.P.
Crystal structure of the bifunctional dihydroneopterin aldolase/6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Streptococcus pneumoniae (2006), J. Mol. Biol., 360, 644-653.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells	Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method with 50 mM MOPS/NaOH (pH 7.0), 17.5% (w/v) methoxy-PEG 2000, 400 mM NaCl, 10 mM MgCl2, 2 mM dithiothreitol, 1 mM EDTA and 5% (v/v) glycerol	Streptococcus pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Mono Q 10/10 column chromatography, Superdex 200 gel filtration	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7,8-dihydroneopterin	-	Streptococcus pneumoniae	6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde	-	?

Subunits

Subunits	Comment	Organism
octamer	x-ray crystallography	Streptococcus pneumoniae
tetramer	active enzyme in solution	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
DHNA-HPPK	DHNA is part of the bifunctional dihydroneopterin aldolase/6 hydroxymethyl-7,8-dihydropterin pyrophosphokinase, also called SulD	Streptococcus pneumoniae

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Release 2023.1 (January 2023)