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Literature summary for 4.1.2.23 extracted from

  • Chou, C.Y.; Ko, T.P.; Wu, K.J.; Huang, K.F.; Lin, C.H.; Wong, C.H.; Wang, A.H.
    Modulation of substrate specificities of D-sialic acid aldolase through single mutations of Val-251 (2011), J. Biol. Chem., 286, 14057-14064.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with hydroxypyruvate, hanging drop vapor diffusion method, using 2.0 M ammonium sulfate and 0.1 M Bis-Tris, pH 6.5 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
199
-
L-arabinose in 50 mM Tris-HCl, pH 7.8, at 37°C Escherichia coli
181
-
N-acetyl-D-mannosamine in 50 mM Tris-HCl, pH 7.8, at 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
sulfate one sulfate ion is found adjacent to the side chain of Lys-165 in the active site and another one on the surface of RS-aldolase Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxy-L-manno-octulosonate
-
Escherichia coli pyruvate + L-arabinose
-
r
D-sialic acid the RS-aldolase has a 5.8fold higher cleavage rate toward 3-deoxy-L-manno-octulosonate than toward D-sialic acid Escherichia coli N-acetyl-D-mannosamine + pyruvate
-
r
N-acetyl-D-mannosamine + pyruvate
-
Escherichia coli D-sialic acid
-
r
pyruvate + L-arabinose
-
Escherichia coli 3-deoxy-L-manno-octulosonate
-
r

Synonyms

Synonyms Comment Organism
RS-aldolase
-
Escherichia coli
L-3-deoxy-manno-2-octulosonic acid aldolase formerly Escherichia coli
L-KDO aldolase formerly Escherichia coli
D-sialic acid aldolase mutant V251I the mutant shows modified substrate specificity from D-sialic acid to 3-deoxy-L-manno-octulosonate Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
N-acetyl-D-mannosamine in 50 mM Tris-HCl, pH 7.8, at 37°C Escherichia coli
7.3
-
L-arabinose in 50 mM Tris-HCl, pH 7.8, at 37°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
L-arabinose in 50 mM Tris-HCl, pH 7.8, at 37°C Escherichia coli
0.0055
-
N-acetyl-D-mannosamine in 50 mM Tris-HCl, pH 7.8, at 37°C Escherichia coli