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Literature summary for 4.1.2.22 extracted from

  • Glenn, K.; Smith, K.S.
    Allosteric regulation of Lactobacillus plantarum xylulose 5-phosphate/fructose 6-phosphate phosphoketolase (Xfp) (2015), J. Bacteriol., 197, 1157-1163 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
glyoxylate 7.2% residual activity at 8 mM Lactiplantibacillus plantarum
additional information the enzyme is unaffected by the presence of ATP, AMP, pyruvate, and acetate Lactiplantibacillus plantarum
oxaloacetic acid about 30% residual activity at 15 mM Lactiplantibacillus plantarum
phosphoenolpyruvate less than 20% residual activity at 15 mM Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.8
-
 D-fructose 6-phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Fructose 6-phosphate + phosphate Lactiplantibacillus plantarum
-
 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate Lactiplantibacillus plantarum
-
 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Fructose 6-phosphate + phosphate
-
 Lactiplantibacillus plantarum Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate
-
 Lactiplantibacillus plantarum Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
Xfp bifunctional enzyme with xylulose 5-phosphate and fructose 6-phosphate phosphoketolase activities Lactiplantibacillus plantarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.05
-
 D-fructose 6-phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum
1.12
-
 phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Lactiplantibacillus plantarum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.93
-
 at pH 6.0 and 37°C Lactiplantibacillus plantarum glyoxylate
6.7
-
 at pH 6.0 and 37°C Lactiplantibacillus plantarum phosphoenolpyruvate
10.5
-
 at pH 6.0 and 37°C Lactiplantibacillus plantarum oxaloacetic acid

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 D-fructose 6-phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum
1.11
-
 phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum