Literature summary for 4.1.2.22 extracted from

Petrareanu, G.; Balasu, M.; Vacaru, A.; Munteanu, C.; Ionescu, A.; Matei, I.; Szedlacsek, S.
Phosphoketolases from Lactococcus lactis, Leuconostoc mesenteroides and Pseudomonas aeruginosa Dissimilar sequences, similar substrates but distinct enzymatic characteristics (2014), Appl. Microbiol. Biotechnol., 98, 7855-7867 .

Search for more literature for 4.1.2.22

Cloned(Commentary)

Cloned (Comment)	Organism
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli	Leuconostoc mesenteroides subsp. mesenteroides
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli	Pseudomonas aeruginosa
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli	Lactococcus lactis subsp. lactis
expressed in Escherichia coli BL21(DE3)RIL cells	Pseudomonas aeruginosa
expressed in Escherichia coli BL21(DE3)RIL cells	Lactococcus lactis
expressed in Escherichia coli BL21(DE3)RIL cells	Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.035	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
0.035	-	D-xylulose 5-phosphate	at pH 6.5 and 37°C	Pseudomonas aeruginosa
1.76	-	D-fructose 6-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
1.76	-	D-fructose 6-phosphate	at pH 6.5 and 37°C	Pseudomonas aeruginosa
2.02	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
2.02	-	D-xylulose 5-phosphate	at pH 6.5 and 37°C	Leuconostoc mesenteroides
3.04	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
3.04	-	D-xylulose 5-phosphate	at pH 6.5 and 37°C	Lactococcus lactis
9.89	-	D-fructose 6-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
9.89	-	D-fructose 6-phosphate	at pH 6.5 and 37°C	Lactococcus lactis
45.96	-	D-fructose 6-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
45.96	-	D-fructose 6-phosphate	at pH 6.5 and 37°C	Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-Fructose 6-phosphate + phosphate	Pseudomonas aeruginosa	-	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	Lactococcus lactis	-	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	Leuconostoc mesenteroides	-	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	Pseudomonas aeruginosa	preferred substrate	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	Lactococcus lactis	preferred substrate	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	Leuconostoc mesenteroides	preferred substrate	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis	-	-	-
Lactococcus lactis subsp. lactis	Q9CFH4	-	-
Lactococcus lactis subsp. lactis IL1403	Q9CFH4	-	-
Leuconostoc mesenteroides	-	-	-
Leuconostoc mesenteroides subsp. mesenteroides	-	-	-
Leuconostoc mesenteroides subsp. mesenteroides J18	-	-	-
Pseudomonas aeruginosa	-	-	-
Pseudomonas aeruginosa	Q9HY13	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa
-	Lactococcus lactis
-	Leuconostoc mesenteroides
-	Leuconostoc mesenteroides subsp. mesenteroides
-	Lactococcus lactis subsp. lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Fructose 6-phosphate + phosphate	-	Pseudomonas aeruginosa	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	-	Lactococcus lactis	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	-	Leuconostoc mesenteroides	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Lactococcus lactis subsp. lactis	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate	Leuconostoc mesenteroides subsp. mesenteroides	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate	Pseudomonas aeruginosa	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Lactococcus lactis subsp. lactis IL1403	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate	Leuconostoc mesenteroides subsp. mesenteroides J18	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	preferred substrate	Pseudomonas aeruginosa	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	preferred substrate	Lactococcus lactis	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	preferred substrate	Leuconostoc mesenteroides	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Leuconostoc mesenteroides subsp. mesenteroides	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Lactococcus lactis subsp. lactis	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose6-phosphate	Pseudomonas aeruginosa	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Lactococcus lactis subsp. lactis IL1403	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Leuconostoc mesenteroides subsp. mesenteroides J18	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 89200, SDS-PAGE	Pseudomonas aeruginosa
?	x * 91858, calculated from amino acid sequence	Leuconostoc mesenteroides
?	x * 94200, SDS-PAGE	Leuconostoc mesenteroides
?	x * 95700, SDS-PAGE	Lactococcus lactis

Synonyms

Synonyms	Comment	Organism
X5P/F6P PK	-	Pseudomonas aeruginosa
X5P/F6P PK	-	Lactococcus lactis
X5P/F6P PK	-	Leuconostoc mesenteroides
XFPK	-	Pseudomonas aeruginosa
XFPK	-	Lactococcus lactis
XFPK	-	Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Pseudomonas aeruginosa
37	-	-	Lactococcus lactis
37	-	-	Leuconostoc mesenteroides

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	40	the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C	Pseudomonas aeruginosa
30	40	the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C	Lactococcus lactis
30	40	the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C	Leuconostoc mesenteroides

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
13.05	-	D-fructose 6-phosphate	at pH 6.5 and 37°C	Pseudomonas aeruginosa
13.1	-	D-fructose 6-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
13.18	-	D-xylulose 5-phosphate	at pH 6.5 and 37°C	Pseudomonas aeruginosa
13.2	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
44.2	-	D-fructose 6-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
44.23	-	D-fructose 6-phosphate	at pH 6.5 and 37°C	Leuconostoc mesenteroides
117.76	-	D-fructose 6-phosphate	at pH 6.5 and 37°C	Lactococcus lactis
117.8	-	D-fructose 6-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
431.78	-	D-xylulose 5-phosphate	at pH 6.5 and 37°C	Leuconostoc mesenteroides
431.8	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
587.76	-	D-xylulose 5-phosphate	at pH 6.5 and 37°C	Lactococcus lactis
587.8	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Lactococcus lactis
7	-	-	Pseudomonas aeruginosa
7	-	-	Leuconostoc mesenteroides

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Pseudomonas aeruginosa
thiamine diphosphate	-	Lactococcus lactis
thiamine diphosphate	-	Leuconostoc mesenteroides
thiamine diphosphate	-	Leuconostoc mesenteroides subsp. mesenteroides
thiamine diphosphate	-	Lactococcus lactis subsp. lactis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Leuconostoc mesenteroides	calculated from amino acid sequence	-	5

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.96	-	D-fructose 6-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
0.96	-	D-fructose 6-phosphate	at pH 6.5 and 37°C	Leuconostoc mesenteroides
7.43	-	D-fructose 6-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
7.43	-	D-fructose 6-phosphate	at pH 6.5 and 37°C	Pseudomonas aeruginosa
11.9	-	D-fructose 6-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
11.9	-	D-fructose 6-phosphate	at pH 6.5 and 37°C	Lactococcus lactis
193.15	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
193.15	-	D-xylulose 5-phosphate	at pH 6.5 and 37°C	Lactococcus lactis
213.43	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
213.43	-	D-xylulose 5-phosphate	at pH 6.5 and 37°C	Leuconostoc mesenteroides
378.62	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
378.62	-	D-xylulose 5-phosphate	at pH 6.5 and 37°C	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)