Literature summary for 4.1.2.22 extracted from

Suzuki, R.; Kim, B.J.; Shibata, T.; Iwamoto, Y.; Katayama, T.; Ashida, H.; Wakagi, T.; Shoun, H.; Fushinobu, S.; Yamamoto, K.
Overexpression, crystallization and preliminary X-ray analysis of xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from Bifidobacterium breve (2010), Acta Crystallogr. Sect. F, 66, 941-943.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells	Bifidobacterium breve

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.05 mM thiamine diphosphate, 0.25 mM MgCl2, 24% (w/v) PEG 6000 and 0.1 M Bicine pH 9.0, at 20°C	Bifidobacterium breve

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.2	-	phosphate	in 40 mM potassium phosphate pH 6.5, 25 mM MES buffer pH 6.5, 0.02 mM ThDP and 0.1 mM MgCl2, at 22°C	Bifidobacterium breve
9.7	-	D-fructose 6-phosphate	in 40 mM potassium phosphate pH 6.5, 25 mM MES buffer pH 6.5, 0.02 mM ThDP and 0.1 mM MgCl2, at 22°C	Bifidobacterium breve

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Bifidobacterium breve

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
90000	-	6 * 90000, SDS-PAGE	Bifidobacterium breve
92700	-	6 * 92700, calculated from amino acid sequence	Bifidobacterium breve
200000	-	three active peaks appear on gel filtration chromatography with estimated native molecular masses of 200 kDa (homodimer), 350 kDa (homotetramer) and 600 kDa (homohexamer)	Bifidobacterium breve
350000	-	three active peaks appear on gel filtration chromatography with estimated native molecular masses of 200 kDa (homodimer), 350 kDa (homotetramer) and 600 kDa (homohexamer)	Bifidobacterium breve
600000	-	three active peaks appear on gel filtration chromatography with estimated native molecular masses of 200 kDa (homodimer), 350 kDa (homotetramer) and 600 kDa (homohexamer)	Bifidobacterium breve

Organism

Organism	UniProt	Comment	Textmining
Bifidobacterium breve	-	-	-
Bifidobacterium breve 203	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration	Bifidobacterium breve

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 6-phosphate + phosphate	-	Bifidobacterium breve	acetyl phosphate + H2O + erythrose 4-phosphate	-	?
D-fructose 6-phosphate + phosphate	-	Bifidobacterium breve 203	acetyl phosphate + H2O + erythrose 4-phosphate	-	?
D-xylulose 5-phosphate + phosphate	-	Bifidobacterium breve	acetyl phosphate + H2O + D-glyceraldehyde 3-phosphate	-	?
D-xylulose 5-phosphate + phosphate	-	Bifidobacterium breve 203	acetyl phosphate + H2O + D-glyceraldehyde 3-phosphate	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 90000, SDS-PAGE	Bifidobacterium breve
homohexamer	6 * 92700, calculated from amino acid sequence	Bifidobacterium breve

Synonyms

Synonyms	Comment	Organism
X5P/F6P phosphoketolase	-	Bifidobacterium breve
XFPK	-	Bifidobacterium breve
xylulose-5-phosphate/fructose-6-phosphate phosphoketolase	-	Bifidobacterium breve

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
25.7	-	phosphate	in 40 mM potassium phosphate pH 6.5, 25 mM MES buffer pH 6.5, 0.02 mM ThDP and 0.1 mM MgCl2, at 22°C	Bifidobacterium breve
25.7	-	D-fructose 6-phosphate	in 40 mM potassium phosphate pH 6.5, 25 mM MES buffer pH 6.5, 0.02 mM ThDP and 0.1 mM MgCl2, at 22°C	Bifidobacterium breve

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	6.5	using D-fructose 6-phosphate as substrate	Bifidobacterium breve

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	required	Bifidobacterium breve

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Release 2023.1 (January 2023)