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Literature summary for 4.1.2.22 extracted from
- Cloning and characterization of a gene encoding phosphoketolase in a Lactobacillus paraplantarum isolated from Kimchi (2007), J. Microbiol. Biotechnol., 17, 822-829.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21 (DE3) | Lactiplantibacillus paraplantarum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.08 | - |
D-fructose 6-phosphate | in 71 mM sodium fluoride and 24 mM sodium iodoacetate, at 37°C | Lactiplantibacillus paraplantarum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88700 | - |
SDS-PAGE | Lactiplantibacillus paraplantarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus paraplantarum | - |
- |
- |
| Lactiplantibacillus paraplantarum C7 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GSTrap HP affinity column chromatography | Lactiplantibacillus paraplantarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
crude cell extract, at 37°C | Lactiplantibacillus paraplantarum |
| 147.3 | - |
after 23.7fold purification, at 37°C | Lactiplantibacillus paraplantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Fructose 6-phosphate + phosphate | - |
Lactiplantibacillus paraplantarum | Acetyl phosphate + D-erythrose 4-phosphate + H2O | - |
? | |
| D-Fructose 6-phosphate + phosphate | - |
Lactiplantibacillus paraplantarum C7 | Acetyl phosphate + D-erythrose 4-phosphate + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Lactiplantibacillus paraplantarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Lactiplantibacillus paraplantarum |
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Release 2023.1 (January 2023)
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